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Downstream processing of bioproducts [PDF]

Engineering in Life Sciences, 2021
Sonja Berensmeier
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Advances in Downstream Processing of Monoclonal Antibodies [PDF]

Antibodies
Although mAbs have great therapeutic potential, their use in medicine is currently limited by the high cost of their manufacturing. Significant developments in upstream processing technologies have caused downstream processing (DSP) to become the ...
Michał Kołodziej, Dorota Antos
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Ionic-liquid-based approaches to improve biopharmaceuticals downstream processing and formulation [PDF]

Frontiers in Bioengineering and Biotechnology, 2023
The emergence of biopharmaceuticals, including proteins, nucleic acids, peptides, and vaccines, revolutionized the medical field, contributing to significant advances in the prophylaxis and treatment of chronic and life-threatening diseases.
Catarina Almeida, Augusto Q. Pedro, Ana P. M. Tavares, Márcia C. Neves, Mara G. Freire   +4 more
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Recent Advances and Future Directions in Downstream Processing of Therapeutic Antibodies [PDF]

International Journal of Molecular Sciences, 2022
Allan Matte
exaly   +2 more sources

Downstream processing of recombinant human insulin and its analogues production from E. coli inclusion bodies

Bioresources and Bioprocessing, 2021
The Global Diabetes Compact was launched by the World Health Organization in April 2021 with one of its important goals to increase the accessibility and affordability of life-saving medicine—insulin.
Yin Yin Siew, Wei Zhang
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Effective flow-through polishing strategies for knob-into-hole bispecific antibodies

Bioresources and Bioprocessing, 2022
Bispecific antibodies (bsAbs), though possessing great therapeutic potential, are extremely challenging to obtain at high purity within a limited number of scalable downstream processing steps.
Serene W. Chen, Kong Meng Hoi, Farouq Bin Mahfut, Yuansheng Yang, Wei Zhang   +4 more
doaj   +1 more source

Excellent removal of knob-into-hole bispecific antibody byproducts and impurities in a single-capture chromatography

Bioresources and Bioprocessing, 2022
Bispecific antibodies (bsAbs) are therapeutically promising due to their ability to bind to two different antigens. However, the bsAb byproducts and impurities, including mispaired homodimers, half-antibodies, light chain mispairings, antibody fragments ...
Serene W. Chen, Kong Meng Hoi, Farouq Bin Mahfut, Yuansheng Yang, Wei Zhang   +4 more
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Investigation of the effect of salt additives in Protein L affinity chromatography for the purification of tandem single-chain variable fragment bispecific antibodies

mAbs, 2020
Tandem single-chain variable fragment (scFv) bispecific antibodies (bsAb) are one of the most promising bsAb formats reported thus far. Yet, because of their increased aggregation propensity, high impurity content due to low expression level, smaller ...
Serene W. Chen, Darryl Tan, Yuan Sheng Yang, Wei Zhang   +3 more
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Enhanced lipase recovery through RSM integrated differential evolutionary approach from the fermented biomass

Brazilian Archives of Biology and Technology, 2013
The aim of this work was to apply a modeling integrated optimisation approach for a complex, highly nonlinear system for an extracellular lipase extraction process. The model was developed using mutation, crossover and selection variables of Differential
Vijay Kumar Garlapati, Pandu Ranga Vundavilli, Rintu Banerjee   +2 more
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Harnessing ceramic hydroxyapatite as an effective polishing strategy to remove product- and process-related impurities in bispecific antibody purification

Bioresources and Bioprocessing, 2023
Bispecific antibody (bsAb), a novel therapeutic modality, provides excellent treatment efficacy, yet poses numerous challenges to downstream process development, which are mainly due to the intricate diversity of bsAb structures and impurity profiles ...
Nattha Ingavat, Xinhui Wang, Jia Min Liew, Farouq Bin Mahfut, Ka Pui But, Yee Jiun Kok, Xuezhi Bi, Yuansheng Yang, Kobayashi Shintaro, Maria Tsoumpra, Wei Zhang   +10 more
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