Results 151 to 160 of about 3,733,624 (286)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Protocol for the generation, viability assessment, and downstream processing of murine precision-cut skin slices. [PDF]

STAR Protoc
Perrard N, Jendoubi M, Daunou B, Mana F, Largy A, Danes M, Dubuquoy L, Dubucquoi S, Launay D, Speca S.   +9 more
europepmc   +1 more source

Membrane Chromatography-Based Downstream Processing for Cell-Culture Produced Influenza Vaccines. [PDF]

Vaccines (Basel), 2022
Yang Z, Xu X, Silva CAT, Farnos O, Venereo-Sanchez A, Toussaint C, Dash S, González-Domínguez I, Bernier A, Henry O, Kamen A.   +10 more
europepmc   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

From Microalgal Biomass to Products: Downstream Processing Technology Gaps and the Road to Commercial Diversification. [PDF]

Microorganisms
Peest TM, Stellner NI, Viswanathan S, Lau R, Garbe D, Brueck TB.   +5 more
europepmc   +1 more source

Optimization of the production process for the anticancer lead compound illudin M: downstream processing. [PDF]

Microb Cell Fact, 2022
Chaverra-Muñoz L, Briem T, Hüttel S.
europepmc   +1 more source

Toward microfluidic continuous-flow and intelligent downstream processing of biopharmaceuticals

<jats:p>The concept of miniaturized biopharmaceutical downstream processing with AI-controlled continuous flow platforms is described to overcome limitations of conventional processes, potentially accelerating the development of novel ...
Mottafegh, Amirreza, Joo, Jeong-Un, Kang, Ji-Ho, Wang, Lei, kim, dong-pyo, Sharma, Vikas   +5 more
core   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

FEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard, Emma‐Jayne Proctor, Jiawa Wang, Nikolas P. Johnston, Andrew Hayes, Georgia McCorkell, Haibo Yu, Jai Tree, Mark R. Davies, Mark J. Walker, Ronald Sluyter, Stephan Brouwer, Martina L. Sanderson‐Smith   +12 more
wiley   +1 more source

Introduction and clearance of beta-glucan in the downstream processing of monoclonal antibodies. [PDF]

Biotechnol Prog, 2021
Kluters S, Steinhauser K, Pfänder R, Studts J.   +3 more
europepmc   +1 more source