Results 81 to 90 of about 336,267 (214)

Sheath‐assisted microfluidics for elasto‐inertial manipulation of micro/nanoparticles: From theory to biomedical applications

Droplet
Bioparticle manipulation is vital for diagnostics, therapeutics, and biological research. Microfluidic technology provides high throughput and precise control, yet traditional methods using Newtonian fluids face challenges like cell damage due to high ...
Huan Wang, Yanting Wang, Yibo Cui, Limiao Yin, Jinwen Li, Yanxuan Wu, Piwu Li, Lei Zhao, Xiaowen Huang   +8 more
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Recent advances in boosting interfacial bubble dynamics with external fields

Droplet
The efficiency of industrial electrocatalytic reactions depends not only on generating gaseous products but also on their detachment from the catalyst surface.
Wenlu Xie, Yanwei Zhang, Yi Liu, Yiming Wei, Jing‐Li Luo, Chenyu Xu   +5 more
doaj   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Silicone oil–based ferrofluid microrobots for versatile magnetic digital microfluidics

Droplet
Magnetic digital microfluidics offers a promising alternative to the dominant electrowetting‐based digital microfluidics but faces significant challenges regarding system portability, sample contamination, and limited functionality.
Pu Liu, Leilei Chen, Yulei Fu, Wendong Wang   +3 more
doaj   +1 more source

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

FEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose, Kotaro Ozaki, Ryohei Kuriyama, Yuka Ikemoto, Kazuhiro Mio, Yuji C. Sasaki, Tatsuya Arai, Sakae Tsuda, Yoichi Shinkai, Masahiro Kuramochi   +9 more
wiley   +1 more source

Droplet microfluidics: Enabling the tailored fabrication of hydrogel particles

Droplet
Hydrogel particles are attracting increasing interest as tunable microscale platforms that combine precise architectures with diverse chemical, biological, and physical functionalities. Among fabrication strategies, droplet microfluidics has emerged as a
Zhongqiao Gan, Xu Hou
doaj   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Effects of IGFBP4 deficiency on human preadipocyte proliferation and differentiation through the IGF1R/AKT pathway

FEBS Open Bio, EarlyView.
IGFBP4 knockdown (KD) impairs preadipocyte proliferation and is associated with IGF1R protein downregulation and attenuated AKT phosphorylation. The mechanisms by which IGFBP4 KD influences the IGF1R/AKT signaling pathway involve newly synthesized proteins and lysosomal degradation pathways. Created in BioRender.
Yujia Guo, Sandy Richter, Christoph Bach, Wieland Kiess, Anna Kirstein, Diana Le Duc, Antje Garten   +6 more
wiley   +1 more source

Acoustic micro‐beam vortex generator for flow actuation inside droplets

Droplet
Controlling acoustic streaming inside a droplet has excellent potential for enabling fluid and particle operations such as mixing, separation, and aggregation in various applications.
Diego Sánchez‐Saldaña, Maria Fernandino, Carlos A. Dorao   +2 more
doaj   +1 more source