ATAC‐seq in Emerging Model Organisms: Challenges and Strategies
Principle of ATAC‐seq (left), overview of arthropod species with published ATAC‐seq data (middle), summary of experimental design (right). ABSTRACT The Assay for Transposase‐Accessible Chromatin with sequencing (ATAC‐seq) is a versatile and widely utilized method for identifying potential regulatory regions, such as promoters and enhancers, within a ...
Duğçar Ebrar Erdoğan+13 more
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Lamin variants cause cardiac arrhythmogenicity in Drosophila.
van Wijk SW+7 more
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E-cadherin endocytosis promotes non-canonical EGFR:STAT signalling to induce cell death and inhibit heterochromatinisation. [PDF]
Ramirez Moreno M+5 more
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Calpain Proteases and the Evolving Signaling Network in Insect Embryonic Patterning
Insect embryonic Dorsal‐Ventral (DV) patterning relies on the BMP and Toll pathways to different extents. Calcium‐dependent cystein proteases of the Calpain family also exert an important function to pattern the DV axis. In Drosophila, Calpain A cleaves the Cactus/IkappaB inhibitor and modifies Toll signals in ventral regions of the embryo. In Rhodnius
Alison Julio, Helena Araujo
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Executioner caspase is proximal to Fasciclin 3 which facilitates non-lethal activation in <i>Drosophila</i> olfactory receptor neurons. [PDF]
Muramoto M+5 more
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Natural SEL1L variants rescue a model of NGLY1 deficiency and modify ERAD function and proteasome sensitivity. [PDF]
Tu'ifua TK, Chow CY.
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ABSTRACT Aedes aegypti, a critical vector for tropical diseases, poses significant challenges for studying its embryogenesis due to difficulties in removing its rigid chorion and achieving effective fixation for in situ hybridization. Here, we present novel methodologies for fixation, dechorionation, DAPI staining, and in situ hybridization, enabling ...
Renata Coutinho‐dos‐Santos+8 more
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Binding of Transcriptional Activator to Silent Loci Causes Their Detachment from the Nuclear Lamina in <i>Drosophila</i> Neurons and Salivary Gland Cells. [PDF]
Simonov RA+6 more
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Recently Evolved, Stage‐Specific Genes Are Enriched at Life‐Stage Transitions in Flies
We discovered that 20%–30% of protein‐coding genes in Drosophila melanogaster and Aedes aegypti are only expressed during specific stages of development. Surprisingly, many of these stage‐specific genes are newly evolved, unique to flies and mosquitoes, shedding light on how insect development has evolved over time.
Andreas Remmel+3 more
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