Results 51 to 60 of about 15,531 (235)

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Relationship between temporal dynamics of intrinsic brain activity and motor function remodeling in patients with acute BGIS

Frontiers in Neuroscience, 2023
Backgroundpatients with acute basal ganglia ischemic stroke (BGIS) show changes in local brain activity represented by the amplitude of low-frequency fluctuation (ALFF), but the time-varying characteristics of this local nerve activity are still unclear.
Xiaoling Chen, Wenmei Li
doaj   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

FEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong, Yuhei Hosokawa, Lars Oliver Essen, Manuel Maestre‐Reyna   +3 more
wiley   +1 more source

Static and temporal dynamic changes of intrinsic brain activity in early-onset and adult-onset schizophrenia: a fMRI study of interaction effects

Frontiers in Neurology
BackgroundSchizophrenia is characterized by altered static and dynamic spontaneous brain activity. However, the conclusions regarding this are inconsistent.
Jingli Chen, Jingli Chen, Jingli Chen, Yarui Wei, Yarui Wei, Kangkang Xue, Kangkang Xue, Xinyu Gao, Xinyu Gao, Mengzhe Zhang, Mengzhe Zhang, Shaoqiang Han, Shaoqiang Han, Baohong Wen, Baohong Wen, Guangyu Wu, Jingliang Cheng, Jingliang Cheng   +17 more
doaj   +1 more source

Pharmacological inhibition of the PERK pathway modulates hepatocellular carcinoma growth and immune signaling

FEBS Open Bio, EarlyView.
Pharmacological inhibition of PERK in a DEN‐induced mouse model of liver cancer does not reduce tumor burden but alters cellular stress signaling. Despite blocking PERK activity, downstream stress responses, including CHOP expression, remain active, suggesting compensatory mechanisms within the unfolded protein response that may influence tumor ...
Ada Lerma‐Clavero, Maria Kopsida, Nathalie Arendt, Hans Lennernäs, Markus Sjöblom, Femke Heindryckx   +5 more
wiley   +1 more source

Optimizing photoexcitation conditions for time‐resolved X‐ray solution scattering experiments

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering (TR‐XSS) is a powerful technique to visualize how proteins change their structure in real time after light activation. Selecting the right laser photoexcitation conditions—fluence, excitation geometry, and sample refresh rate—is critical to maximize the experimental signal while avoiding unwanted side effects ...
Matteo Levantino
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Speed sensorless sliding mode control of induction motor based on genetic algorithm optimization

Measurement + Control, 2020
In the original model reference adaptive induction motor speed sensorless system based on flux linkage, there is a large fluctuation of the rotational speed in transient and steady state.
Haijun Che, Binglin Wu, Jingming Yang, Ying Tian   +3 more
doaj   +1 more source

Amplitude of low-frequency fluctuation after a single-trigger pain in patients with classical trigeminal neuralgia

The Journal of Headache and Pain, 2022
Objective This study aimed to explore the central mechanism of classical trigeminal neuralgia (CTN) by analyzing the static amplitude of low-frequency fluctuation (sALFF) and dynamic amplitude of low-frequency fluctuation (dALFF) in patients with CTN ...
Xiuhong Ge, Luoyu Wang, Lei Pan, Haiqi Ye, Xiaofen Zhu, Sandra Fan, Qi Feng, Wenhua Yu, Zhongxiang Ding   +8 more
doaj   +1 more source

Impact of anesthesia on static and dynamic functional connectivity in mice

NeuroImage, 2021
A few studies have compared the static functional connectivity between awake and lightly anesthetized states in rodents by resting-state fMRI. However, impact of light anesthesia on static and dynamic fluctuations in functional connectivity has not been ...
Tomokazu Tsurugizawa, Daisuke Yoshimaru
doaj   +1 more source