Results 61 to 70 of about 12,139,664 (308)
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace +6 more
wiley +1 more source
Proteostasis and the gut microbiota play a key role in shaping host physiology. Microbiota‐derived metabolites, vitamins, and RNA modulate host proteostasis. Findings from model systems, including C. elegans, indicate microbes can either stabilize or disrupt host proteostasis.
Abhishek Anil Dubey, Maria Ermolaeva
wiley +1 more source
As a widely used structural material in construction, the energy dissipation characteristics of concrete under dynamic impact are crucial for evaluating a structure’s impact resistance and safety performance.
Xiaoyu Jia +4 more
doaj +1 more source
Design and analysis strategies for robust microbiome ageing research
The gut microbiome changes with age and associates with age‐related morbidity and mortality, establishing it as a potential biomarker and intervention target for ageing. Realising this potential requires methodological rigour, yet distinguishing biological signals from methodological artefacts remains challenging across cohorts. This review provides an
Mark Olenik +5 more
wiley +1 more source
Reconstructing enzyme evolution by protein engineering
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler +2 more
wiley +1 more source
The dynamic viscoelastic characterisation of the impact behaviour of the GAA sliotar [PDF]
In recent years variability in behaviour of the sliotar, a small leather-bound ball used in the Irish sport of hurling, became evident in championship matches. The current standard has not provided adequate repeatability of ball performance. A new method
Kieran Moran +5 more
core +2 more sources
High-strength steel T-joints are widely used in the load-bearing components of critical engineering equipment such as ships, offshore platforms, and rail transit.
Jianhang GUO +4 more
doaj +1 more source
The control of broken rock mass roadways has always been a difficult problem in metal hard rock mines. In this paper, the mechanical mechanism of anchor net support is characterized and simulated through the physical model test of the prestressed support
Shaolong Qin +3 more
doaj +1 more source
Investigating transcription factor dynamics in health and disease using FRAP
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj +3 more
wiley +1 more source

