Results 191 to 200 of about 2,028 (216)

Extrachromosomal DNA (ecDNA) in cancer pathogenesis

Current Opinion in Genetics & Development, 2021
In cancer, oncogenes and surrounding regulatory regions can untether themselves from chromosomes, forming extrachromosomal DNA particles (ecDNAs). Because of their non-chromosomal inheritance, ecDNA drives high oncogene copy number and intratumoral genetic heterogeneity, endowing tumors with the ability to rapidly change their genomes, accelerating ...
Sihan Wu, Vineet Bafna, Paul S Mischel
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Immortalization and transformation of primary cells mediated by engineered ecDNAs

2023
ABSTRACTFocal gene amplifications are among the most common cancer-associated mutations, but their evolution and contribution to tumorigenesis have proven challenging to recapitulate in primary cells and model organisms. Here we describe a general approach to engineer large (>1 Mbp) focal amplifications mediated by extrachromosomal circular DNAs ...
Davide Pradella, Minsi Zhang, Rui Gao, Melissa A. Yao, Katarzyna M. Gluchowska, Ylenia Cendon Florez, Tanmay Mishra, Gaspare La Rocca, Moritz Weigl, Ziqi Jiao, Hieu H.M. Nguyen, Felix Grimm, Marta Lisi, Chiara Mastroleo, Kevin Chen, Jens Luebeck, Vineet Bafna, Cristina R. Antonescu, Andrea Ventura   +18 more
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Impact of ecDNA: A mechanism that directs tumorigenesis in cancer drug Resistance-A review

Chemico-Biological Interactions, 2022
Extrachromosomal DNA (ecDNA) is often found in cancerous cells, and numerous scientific investigations have already shown that ecDNA-mediated oncogene amplification which contributes to cancer therapy resistance. This ecDNA is found to be essential for enhancing gene transcription and resistance to chemotherapeutic drugs, as well as promoting tumor ...
Sumel, Ashique, Aakash, Upadhyay, Ashish, Garg, Neeraj, Mishra, Afzal, Hussain, Poonam, Negi, Goh Bey, Hing, Shvetank, Bhatt, Md Khadem, Ali, Kuppusamy, Gowthamarajan, Sachin Kumar, Singh, Gaurav, Gupta, Dinesh Kumar, Chellappan, Kamal, Dua   +13 more
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Abstract 1626: Tumors driven by oncogene amplified extrachromosomal DNA (ecDNA) demonstrate enhanced sensitivity to cell cycle checkpoint kinase 1 (CHK1) inhibition

Cancer Research, 2023
Patients whose tumors harbor oncogene amplification on extrachromosomal DNA (ecDNA) fail to respond to targeted or immune therapy and have poor prognosis.
S. Chowdhry, S. Milutinovic, Edison Tse, Salvador Garcia, Dean Perusse, Melissa Ritland, J. Ko, Deepti Wilkinson, Kristen M. Turner, Auzon Steffy, Joshua Plum, Ben Norman, AnnMarie Pferdekamper, Todd Meyer, D. Liao, Rachelle Elsdon, Joshua T. Lange, A. Pinkerton, Ryan J. Hansen, C. Hassig, S. Kasibhatla   +20 more
semanticscholar   +1 more source

PATH-31. ECDNA PROMOTES TUMORIGENICITY AND INTRATUMORAL HETEROGENEITY IN MEDULLOBLASTOMA

Neuro-Oncology, 2022
Abstract Extrachromosomal circular DNA (ecDNA) is an important driver of aggressive cancers, including medulloblastoma (MB), the most common pediatric brain tumor. To assess the clinical importance of ecDNA in MB, we applied computational methods to detect ecDNA in the genomes of a cohort of 468 MB patients and 31 MB model systems. Among
Owen Chapman, Sunita Sridhar, Shanqing Wang, Jill Mesirov, Lukas Chavez   +4 more
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Abstract B082: A novel, potent and selective ribonucleotide reductase (RNR) inhibitor, BBI-825, blocks extrachromosomal DNA (ecDNA) amplification-mediated resistance to KRASG12C inhibitor in colorectal cancer (CRC)

Molecular Cancer Therapeutics, 2023
Introduction: KRASG12C inhibitors are a new therapeutic approach to treat KRAS mutant-driven cancers, but as with other oncogene directed therapies, adaptive resistance develops quickly and frequently.
Sudhir Chowdhry, Ryan J. Hansen, Jacques Mauger, A. Pferdekamper, Edison Tse, Ben Norman, Jafar Moininazeri, Salvador Garcia, Yen Truong, Debbie Liao, Joshua Lange, Evan Holmes, Anthony Celeste, Dave Solis, Auzon Steffy, Joshua Plum, Julie Wiese, Julius Apuy, Ardalan Ardeshiri, S. Milutinovic, Deepti Wilkinson, Anthony B Pinkerton, Christian A Hassig, S. Kasibhatla   +23 more
semanticscholar   +1 more source

Abstract B092: Intrinsic genomic plasticity of extrachromosomal DNA (ecDNA) enables oncogene amplified tumor cells to develop rapid acquired resistance to targeted therapy

Molecular Cancer Therapeutics, 2023
Background: Focal high copy number oncogene amplification frequently occurs on extrachromosomal DNA (ecDNA), acentric circular DNA derived from chromosomes during neoplastic transformation and tumor progression.
Kristen Turner, Homa Hemmati, Cory DuPai, John Bibay, Claire Klebs, Joan Chen, Debbie Liao, Christian A Hassig, S. Kasibhatla   +8 more
semanticscholar   +1 more source

Association of ERBB2 extrachromosomal DNA (ecDNA) with first-line HER2-targeted treatment outcomes in advanced esophagogastric adenocarcinoma (EGC).

Journal of Clinical Oncology
4041 Background: ecDNA-enabled amplification of oncogenes is associated with increased expression and poor prognosis and is believed to contribute to treatment resistance.
Charlton Tsai, Shalabh Suman, Cynthia Chu, Joanne F. Chou, P. Krein, M. Capanu, D. Ilson, G. Ku, Y. Janjigian, A. Drilon, Michael F. Berger, S. Maron   +11 more
semanticscholar   +1 more source

Abstract 5870: Taxane-based chemotherapy leads to acquired resistance through ecDNA-based amplification of multi-drug resistance genes

Cancer Research
Focal amplification on extrachromosomal DNA (ecDNA) drives high gene copy number and intercellular genomic heterogeneity, which can fuel tumor adaptation and drug resistance.
Kristen Holmes, Dean Perusse, Cory DuPai, Homa Hemmati, Julie Weiss, Claire Kuelbs, John Bibay, Joan Chen, Debbie Liao, Christian A Hassig, S. Kasibhatla   +10 more
semanticscholar   +1 more source

Shedding Light on ecDNA Behavior Using CRISPR-Based Live-Cell Imaging

Cancer Discovery, 2022
Abstract Summary: Extrachromosomal oncogene amplification on extrachromosomal DNA (ecDNA) has emerged as a hallmark of many cancers. In this issue, Yi and colleagues developed a CRISPR-based method for imaging ecDNA in live cells, termed ecTag.
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