Results 291 to 300 of about 425,088 (366)

Direct Quantification of Protein–Protein Interactions in Living Bacterial Cells

open access: yesAdvanced Science, EarlyView.
Quantitative measurement of protein–protein interactions in living cells is vital for biotechnology applications. Fluorescence resonance energy transfer (FRET) signals in bacterial cells are often weak, leading to false‐positive. Quantitative FRET measurements are implemented to overcome the weak FRET signal.
Soojung Yi   +12 more
wiley   +1 more source

Development of Carbon‐11 Labeled Pyrimidine Derivatives as Novel Positron Emission Tomography (PET) Agents Enabling Brain Sigma‐1 Receptor Imaging

open access: yesAdvanced Science, EarlyView.
Novel carbon‐11 labeled pyrimidine derivatives are developed as PET imaging probes for visualizing sigma‐1 receptors in the brain. [11C]CNY‐01 demonstrates superior brain uptake and binding specificity in both rodents and non‐human primates. The study reveals decreased sigma‐1 receptor expression in Alzheimer's disease, offering a promising tool for ...
Ping Bai   +12 more
wiley   +1 more source

Identification of Tumor‐Specific Surface Proteins Enables Quantification of Extracellular Vesicle Subtypes for Early Detection of Pancreatic Ductal Adenocarcinoma

open access: yesAdvanced Science, EarlyView.
Pancreatic ductal adenocarcinoma (PDAC) extracellular vesicle (EV) surface protein assay operates via a two‐step workflow – Step 1: Click chemistry‐mediated enrichment of PDAC EVs by EV Click Beads, in the presence of one of the three trans‐cyclooctene‐grafted PDAC EV‐specific antibodies; Step 2: Quantification of the enriched PDAC EVs by reverse ...
Chen Zhao   +27 more
wiley   +1 more source

High‐Speed Hyperspectral Imaging for Near Infrared Fluorescence and Environmental Monitoring

open access: yesAdvanced Science, EarlyView.
This study introduces a fast hyperspectral imaging method in the near‐infrared range (HyperNIR). Combining tunable polarization optics with a phasor‐based approach, HyperNIR captures detailed spectral information with just three images. The applicability is showcased by distinguishing NIR fluorophores that are separated by 5 nm.
Jan Stegemann   +9 more
wiley   +1 more source

In Vivo Reprogramming of Tissue‐Derived Extracellular Vesicles for Treating Chronic Tissue Injury Through Metabolic Engineering

open access: yesAdvanced Science, EarlyView.
Inspired by exercise‐induced metabolic rewiring and EV secretion, this study shows that in vivo metabolic engineering strategy can reprogram EV secretion pattern and simultaneously attenuate multiple tissue injury in CKD status, highlighting that it is a potential strategy for treating diverse chronic diseases.
Meng Zhao   +11 more
wiley   +1 more source

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