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Volume electron microscopy

open access: yesNature Reviews Methods Primers, 2022
Life exists in three dimensions, but until the turn of the century most electron microscopy methods provided only 2D image data. Recently, electron microscopy techniques capable of delving deep into the structure of cells and tissues have emerged, collectively called volume electron microscopy (vEM).
Constantin Pape   +2 more
exaly   +5 more sources

Biomedical and biological applications of scanning electron microscopy [PDF]

open access: yes, 2012
Podeu consultar el llibre complet a: http://hdl.handle.net/2445/32166This article summarizes the basic principles of scanning electron microscopy and the capabilities of the technique with different examples of applications in biomedical and biological ...
Cortadellas i Raméntol, Núria   +2 more
core   +6 more sources

Transmission electron microscopy in cell biology: sample preparation techniques and image information [PDF]

open access: yes, 2012
Podeu consultar el llibre complet a: http://hdl.handle.net/2445/32166Transmission electron microscopy is a proven technique in the field of cell biology and a very useful tool in biomedical research. Innovation and improvements in equipment together with
Cortadellas i Raméntol, Núria   +2 more
core   +7 more sources

3D solutions in transmission electron (Cryo) -microscopy in biology [PDF]

open access: yes, 2012
Podeu consultar el llibre complet a: http://hdl.handle.net/2445/32166One of the main problems in transmission electron microscopy in the biological field is the tri-dimensionality.
Muela, Yolanda   +4 more
core   +6 more sources

Perspective towards atomic‐resolution imaging of two‐dimensional polymers

open access: yesSmartMat, 2021
Recent years have witnessed the rise of an emerging class of synthetic two‐dimensional (2D) materials‐2D polymers. The combination of organic chemistry and rational design of polymeric crystals has stimulated tremendous research efforts in the controlled
Haoyuan Qi, Baokun Liang, Ute Kaiser
doaj   +1 more source

Multiscale and Correlative Analytical Electron Microscopy of Extraterrestrial Minerals

open access: yesFrontiers in Astronomy and Space Sciences, 2020
This paper presents a unique correlative microscopic method for the structural characterization of extraterrestrial minerals. A fragment from the pallasite Seymchan meteorite that consists of olivine grains mixed into a metallic iron matrix with variable
Mihaela Albu   +6 more
doaj   +1 more source

Precise targeting for 3D cryo-correlative light and electron microscopy volume imaging of tissues using a FinderTOP

open access: yesCommunications Biology, 2023
Cryo-correlative light and electron microscopy (cryoCLEM) is a powerful strategy to high resolution imaging in the unperturbed hydrated state. In this approach fluorescence microscopy aids localizing the area of interest, and cryogenic focused ion beam ...
Marit de Beer   +7 more
doaj   +1 more source

Vaccinia virus H7-protein is required for the organization of the viral scaffold protein into hexamers

open access: yesScientific Reports, 2022
Viruses of the giant virus family are characterized by a structurally conserved scaffold-capsid protein that shapes the icosahedral virion. The vaccinia virus (VACV) scaffold protein D13, however, transiently shapes the newly assembled viral membrane in ...
Susanne Tonnemacher   +8 more
doaj   +1 more source

Methods for studying the localization of mitochondrial complexes III and IV by immunofluorescent and immunogold microscopy [PDF]

open access: yesArchives of Biological Sciences, 2016
The localization of proteins within a cell is very important for studying protein colocalization and subsequently understanding protein-protein interactions at the subcellular level.
Golić Igor   +5 more
doaj   +1 more source

Electron Microscopy [PDF]

open access: yes, 2012
Correlative light (LM) and transmission electron microscopic (TEM) analysis is useful, if ultrastructural details of cells need to be related to functional aspects which can only be examined at the LM level. The first protocol presented here introduces a relatively simple way of obtaining TEM images which, on the one hand, reveal ultrastructural ...
Luder, H U, Amstad-Jossi, M
openaire   +3 more sources

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