Results 211 to 220 of about 26,042 (268)
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Sephadex sandwich disc electrophoresis
Analytical Biochemistry, 1970Abstract A new Sephadex sandwich disc electrophoresis method has been devised for effective resolution of complex macromolecules on the basis of their charge and molecular dimensions. As evidenced by separation of mitochondrial and serum lipoproteins from contaminating proteins, superior resolution of these macromolecules is obtained with this ...
Sreenivasan A +2 more
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Monoclonal immunoglobulins on disc electrophoresis
Klinische Wochenschrift, 1972A transparent upper gel disc electrophoretic technique was applied for the detection of mono- and oligo-clonal immunoglobulins. This method allows the visualization and densitometrical recording of those proportions of IgG and IgM retained in the upper concentration gel.
K Felgenhauer, G Alzer, K Schumacher
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A microgel system for disc electrophoresis
Analytical Biochemistry, 1976Abstract Disc electrophoresis has provided a suitable method for separating macromolecules ( Davis, 1964 ; Ornstein, 1964 ). Numerous authors (2,5–10, 12) have presented scaled-down versions which were not directly comparable to the standard separations.
Natalie Tulchin +2 more
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Immunoglobulins in disc electrophoresis
Clinica Chimica Acta, 1972Abstract With the widely used disc electrophoresis technique of Ornstein and Davis, considerable part of the immunoglobulins is retained within the turbid concentration gel. A modification of this technique is described which allows better evaluation of these retained immunoglobulins. Using a modified concentration gel the bulk of IgG and all IgM are
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Quantitative immun oassay by disc electrophoresis
Clinica Chimica Acta, 1967Abstract A method is described whereby the extent of interaction between antigen and purified antibody solutions can be quantitatively determined. An immune globulin solution, prepared from horse antisera to human albumin, was admixed with largepore spacer gel in polyacrylam ide disc electrophoresis.
William F. Blatt, Robert Louis-Ferdinand
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Proteins, Persulphate and Disc Electrophoresis [PDF]
Nature, 1967 POLYACRYLAMIDE electrophoresis of purified chick interferon at pH 8.9 by methods similar to that of Davis1 gave an average recovery of activity of about 30 per cent2,3. The recovery could be improved by omitting the spacer and sample gels. Electrophoresis at pH.
I. G. S. Furminger, K. H. Fantes
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Disc electrophoresis: an improved technique.
The Journal of laboratory and clinical medicine, 1966Abstract The disc electrophoretic technique of Ornstein and Davis has been modified to eliminate the need of rubber serum bottle stoppers and to facilitate the use of fluid, unpolymerized, samples. The basis of the new technique is a specially designed apparatus which holds and seals the ends of the tubes during filling and polymerization.
Ann Hale, Thomas E. Nelson
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A simple apparatus for disc electrophoresis
Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis, 1965Abstract 1. 1. After a brief review of the development of methods for disc electrophoresis on polyacrylamide gel the construction of a simple and robust apparatus for disc electrophoresis is described. 2. 2. Although the basis of design and operation is similar to previously used methods the present apparatus has certain constructional ...
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Disc electrophoresis of mycobacterial sensitins.
The American review of respiratory disease, 1969A cell extract of Mycobacterium kansasii was subjected to polyacrylamide electrophoresis. At least ten bands could be distinguished and four major zones were separated. Chemical analysis and enzymatic degradation of the material eluted from the four major zones indicated that skin-test activity was correlated with polysaccharide, possibly in associa ...
Stevan Marcus +2 more
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Analytical Biochemistry, 1966
A device for quick destaining of polyacrylamide gel cylinders after staining with amido black has been described. The apparatus was also used to investigate the possibility of short-time staining and electrophoretic staining, permitting rapid orientation regarding protein distribution on the gel.
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A device for quick destaining of polyacrylamide gel cylinders after staining with amido black has been described. The apparatus was also used to investigate the possibility of short-time staining and electrophoretic staining, permitting rapid orientation regarding protein distribution on the gel.
openaire +3 more sources