Results 251 to 260 of about 63,842 (308)

Sephadex sandwich disc electrophoresis

Analytical Biochemistry, 1970
Abstract A new Sephadex sandwich disc electrophoresis method has been devised for effective resolution of complex macromolecules on the basis of their charge and molecular dimensions. As evidenced by separation of mitochondrial and serum lipoproteins from contaminating proteins, superior resolution of these macromolecules is obtained with this ...
S V, Koppikar, P, Fatterpaker, A, Sreenivasan   +2 more
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DISC ELECTROPHORESIS OF IRRADIATED TRYPSIN*

Photochemistry and Photobiology, 1966
Abstract— Trypsin inactivated by u.v. radiation, gamma radiation, visible radiation in the presence of sensitizing dyes, and autolysis, was examined by the method of disc electrophoresis. Untreated Worthington twice crystallized salt‐free trypsin separated into four bands which moved toward the cathode; the main band, which had the greatest mobility ...
, Ghironca, A F, Ghiron, J D, Spikes
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Monoclonal immunoglobulins on disc electrophoresis

Klinische Wochenschrift, 1972
A transparent upper gel disc electrophoretic technique was applied for the detection of mono- and oligo-clonal immunoglobulins. This method allows the visualization and densitometrical recording of those proportions of IgG and IgM retained in the upper concentration gel.
K, Felgenhauer, G, Alzer, K, Schumacher
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Quantitative immun oassay by disc electrophoresis

Clinica Chimica Acta, 1967
Abstract A method is described whereby the extent of interaction between antigen and purified antibody solutions can be quantitatively determined. An immune globulin solution, prepared from horse antisera to human albumin, was admixed with largepore spacer gel in polyacrylam ide disc electrophoresis.
R, Louis-Ferdinand, W F, Blatt
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Immunoglobulins in disc electrophoresis

Clinica Chimica Acta, 1972
Abstract With the widely used disc electrophoresis technique of Ornstein and Davis, considerable part of the immunoglobulins is retained within the turbid concentration gel. A modification of this technique is described which allows better evaluation of these retained immunoglobulins. Using a modified concentration gel the bulk of IgG and all IgM are
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Disc electrophoresis of lactate dehydrogenase isoenzymes

Clinica Chimica Acta, 1970
Abstract The original method of disc electrophoresis permitted the localization of only 4 of the 5 lactate dehydrogenase isoenzymes. Conjecture as to the location of one of these is now settled in that it is demonstrated that the lactate dehydrogenase-5 does not migrate through the stacking gel. This was shown for human serum and liver.
A A, Dietz, T, Lubrano, H M, Rubinstein
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SIMPLIFIED “DISC” (POLYACRYLAMIDE GEL) ELECTROPHORESIS*

Annals of the New York Academy of Sciences, 1964
SummaryCross‐linked homogeneous 5 per cent polyacrylamide gel in rod form is a convenient medium for sharp analytical electrophoretic separation in microgram quantities of most serum proteins and of many biological ampholytes.
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Disc electrophoresis of bovine thymus histones

Comparative Biochemistry and Physiology, 1966
Abstract Polyacrylamide gel disc electrophoresis showed no differences in the thymus histones of Bos bovus of different age, sex or breed.
G R, Shepherd, L R, Gurley
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Simplified Technique for Preparative Disc Electrophoresis

Nature, 1965
THE disc electrophoretic method1 is becoming an important analytical tool in the study of components of complex mixtures. While engaged in work on the separation of antigenic constituents of connective tissue, we did some experiments to adapt this method to a preparative scale. Our aim was to construct as simple and inexpensive an apparatus as possible.
D, Sulitzeanu, W F, Goldman
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