Results 21 to 30 of about 26,042 (268)

Acrylamide gel electrophoresis of proteins, acid phosphatases and RN-ases from three potato varieties

open access: yesActa Societatis Botanicorum Poloniae, 2015
Studies on variety differences in the protein and acid phosphatase patterns as well as ribunuclease activity distribution were carried out by disc electrophoresis on saline extracts of three varieties of the potato Solanum tuberosum (L.).
A. Kubicz, E. Wieczorek, B. Morawiecka
doaj   +1 more source

Isolation and properties of polyphenol oxidase from basidiocarps of Lactarius pergamenus Fr. (Fr.) fungi [PDF]

open access: yesThe Ukrainian Biochemical Journal, 2015
Fresh juice of basidiocarps of Lactarius pergamenus Fr. (Fr.) fungi was subjected to ion exchange chromatography with used DEAE-toyopearl and CM-cellulose columns, as well as preparative electrophoresis in 7.5% polyacrylamide gels (pH 8.6).
M. V. Tsivinska   +2 more
doaj   +1 more source

Apolipoprotein A-II content of human plasma high density lipoproteins measured by radioimmunoassay

open access: yesJournal of Lipid Research, 1977
A double antibody radioimmunoassay for human ApoA-II is reported. ApoA-II isolated from human plasma high density lipoprotein (HDL) by column chromatography migrated as a single band on polyacrylamide disc gel electrophoresis, had the appropriate amino ...
G Schonfeld   +3 more
doaj   +1 more source

Sickle Cell Trait Presenting as Unilateral Proliferative Retinopathy and Macular Thinning in a Pregnant Woman

open access: yesCase Reports in Ophthalmological Medicine, 2021
Background. To report a case of a pregnant woman with sickle cell trait (SCT) who presented with unilateral proliferative sickle cell retinopathy. Case Presentation.
Sepideh Ghods   +7 more
doaj   +1 more source

Vitamin A transport in chicken plasma: isolation and characterization of retinol-binding protein (RBP), prealbumin (PA), and RBP–PA complex

open access: yesJournal of Lipid Research, 1975
Vitamin A-transporting protein in chicken plasma was purified by column chromatography on DEAE-Sephadex and Sephadex G-100; the protein formed a complex of retinol-binding protein (RBP) with prealbumin (PA).
T. Abe, Y. Muto, N. Hosoya
doaj   +1 more source

Geographic and climatic differentiation of electrophoretic forms of esterase, glutamate dehydrogenase and peroxidase in Scots pine tissues

open access: yesActa Societatis Botanicorum Poloniae, 2014
The enzymatic systems have been investigated in individual trees derived from 17 distant localities in Europe and Asia. Esterase and glutamate dehydrogenase polymorphism was determined in extracts from mixture of 50 macrogametophytes, iperoxidase ...
Barbara Kieliszewska-Rokicka
doaj   +1 more source

Thermostable neutral metalloprotease from Geobacillus sp. EA1 does not share thermolysin's preference for substrates with leucine at the P1′ position

open access: yesFEBS Letters, EarlyView.
Knowing how proteases recognise preferred substrates facilitates matching proteases to applications. The S1′ pocket of protease EA1 directs cleavage to the N‐terminal side of hydrophobic residues, particularly leucine. The S1′ pocket of thermolysin differs from EA's at only one position (leucine in place of phenylalanine), which decreases cleavage ...
Grant R. Broomfield   +3 more
wiley   +1 more source

Revealing the structure of land plant photosystem II: the journey from negative‐stain EM to cryo‐EM

open access: yesFEBS Letters, EarlyView.
Advances in cryo‐EM have revealed the detailed structure of Photosystem II, a key protein complex driving photosynthesis. This review traces the journey from early low‐resolution images to high‐resolution models, highlighting how these discoveries deepen our understanding of light harvesting and energy conversion in plants.
Roman Kouřil
wiley   +1 more source

Preliminary studies on ribonucleases from Poa pratensis seeds

open access: yesActa Societatis Botanicorum Poloniae, 2015
Ribonuclease was extracted from Poa pratensis seeds with 0.1 M acetate buffer, pH 5.1, and then precipitated with alcohol. The enzyme was separated into 5 fractions (I-V) after chromatography on DEAE-cellulose at pH 5.1.
Irena Lorenc-Kubis   +1 more
doaj   +1 more source

RAD50 missense variants differentially affect the DNA damage response and mitotic progression

open access: yesFEBS Letters, EarlyView.
RAD50 incorporates into the MRN complex and initiates the DNA damage response. Furthermore, RAD50 promotes mitotic progression. RAD50 missense variants capable of forming an MRN complex supported the DNA damage response and mitotic features to different extents in complementation experiments, indicating these functions are separable and might impact ...
Hanna Redeker   +9 more
wiley   +1 more source

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