Preliminary studies on ribonucleases from Poa pratensis seeds
Ribonuclease was extracted from Poa pratensis seeds with 0.1 M acetate buffer, pH 5.1, and then precipitated with alcohol. The enzyme was separated into 5 fractions (I-V) after chromatography on DEAE-cellulose at pH 5.1.
Irena Lorenc-Kubis+1 more
doaj +1 more source
Structural and Functional Analysis of Calcium Ion Mediated Binding of 5-Lipoxygenase to Nanodiscs. [PDF]
An important step in the production of inflammatory mediators of the leukotriene family is the Ca2+ mediated recruitment of 5 Lipoxygenase (5LO) to nuclear membranes.
Ramakrishnan B Kumar+7 more
doaj +1 more source
A spatial analysis of physiological changes associated with infection of cotyledons of marrow plants with cucumber mosaic virus [PDF]
Changes in host primary metabolism associated with the compatible interaction between cucumber mosaic virus and cotyledons of the marrow plant (Cucurbita pepo L.) have been localized, first by measuring activities of key enzymes in infected and ...
Leegood, R.C.+3 more
core +3 more sources
Chimeric diphtheria toxin–CCL8 cytotoxic peptide for breast cancer management
DTCCL8 is a recombinant fusion toxin that targets cancer cells expressing chemokine receptors. By combining diphtheria toxin with CCL8, DTCCL8 binds to multiple receptors on tumor cells and induces selective cytotoxicity. This strategy enables receptor‐mediated targeting of cancer and may support the development of chemokine‐guided therapeutics ...
Bernardo Chavez+5 more
wiley +1 more source
In human monocytic cells THP‐1, a limited uptake of native—not oxidized—LDL/VLDL induced expression of scavenger receptor A and cellular adhesion. Induction was inhibited by lysosomotropic (WR‐1065) and lipophilic (BHT) antioxidants and by siRNAs against ferritinophagy.
Martina Čierna+4 more
wiley +1 more source
THE DAMAGE OF THE OPTIC NERVE AS THE OUTCOME OF UNINTENTIONAL POISONING BY CLOSANTEL
Background: In recent decades, the problem of toxic damage of the visual system due to poisoning by various medicines has become particularly relevant.
Evgenia Sergeevna Kurakina+3 more
doaj +1 more source
Disc gel electrophoresis of caseins treated with proteolytic and glycolytic enzymes.
Calcium caseinate and purified α s -, β-, and κ-caseins were treated with lysozyme, β-glucosidase, rennin, and trypsin. β-glucosidase caused calcium caseinate and κ-casein to precipitate, as did lysozyme.
W. Mullin, F. Wolfe
semanticscholar +1 more source
Antibiotic susceptibility and high prevalence of extended spectrum beta-lactamase producing Escherichia coli in iranian broilers [PDF]
Extended-spectrum β-lactamase (ESBL) producing Escherichia coli have rapidly spread worldwide and cause serious threats for public health. The study was conducted to determine the antibiotic resistance and characterization of ESBL producing E.
Khoshbakht, R., Raeisi, M., Seifi, S.
core
Advances in Microfluidics and Lab-on-a-Chip Technologies
Advances in molecular biology are enabling rapid and efficient analyses for effective intervention in domains such as biology research, infectious disease management, food safety, and biodefense.
Abate+161 more
core +1 more source
Optically addressable single-use microfluidic valves by laser printer lithography [PDF]
We report the design, fabrication, and characterization of practical optofluidic valves fabricated using laser printer lithography. Valves are opened by directing optical energy from a solid-state laser, with similar power characterisitcs to those used ...
Benito-Lopez, Fernando+5 more
core +1 more source