Results 181 to 190 of about 712,932 (237)

‘Laterally aggregated’ polyacrylamide gels for electrophoresis

ELECTROPHORESIS, 1992
AbstractA new method is described for producing highly porous polyacrylamide matrices: polymerization in presence of a preformed hydrophilic polymer. If a standard mixture of monomers (e.g., 5%T, 4%C) is polymerized in presence of, e.g., polyethylene glycol (PEG) 10 kDa, lateral chain aggregation occurs, with formation of large pore sizes.
Righetti P. G., Caglio S., Saracchi M., Quaroni S.   +3 more
openaire   +3 more sources

Polyacrylamide Gel Electrophoresis of Insulin

Analytical Letters, 2007
Abstract Four kinds of polyacrylamide gel electrophoresis (PAGE) were applied to insulin and peroxynitrite‐treated insulin. The Native‐PAGE had a better resolution than sodium dodecyl sulfate (SDS)‐PAGE, SDS‐urea‐PAGE, and even Tricine‐SDS‐PAGE. Reduction and nonreduction of insulin and peroxynitrite‐treated insulin in Native‐PAGE showed that four ...
Kaixun Huang, Quan Chi
openaire   +2 more sources

Polyacrylamide Gel Electrophoresis of Plasteins

Agricultural and Biological Chemistry, 1974
A peptic hydrolysate of soybean protein was filtered with Sephadex G–25 and was separated approximately into four fractions (I, II, II, and IV in the order of mol. wt.). Fraction II (av. mol. wt: 1043) and III (av. mol. Wt.: 685) were more plastein-productive than others.
Soichi Arai, Shun-Jen Tsai, Michiko Yamashita, Masao Fujimaki   +3 more
openaire   +2 more sources

Preparative polyacrylamide gel electrophoresis

Journal of Chromatography A, 1972
Abstract We have studied several variables affecting the migration and resolution of protein mixtures in preparative polyacrylamide gel electrophoresis. In the system we have used (Uniphor column, L.K.B.) the maximum sample load is 10 mg of protein per cm2 per protein band.
Camillo Secchi, Pier Giorgio Righetti
openaire   +2 more sources

Separation of Colicins by Polyacrylamide Gel Electrophoresis

Journal of Applied Bacteriology, 1972
Summary: Colicins can be separated by polyacrylamide gel electrophoresis, so allowing multiple colicinogeny to be detected with a universal indicator strain.
H. C. Neimark, K. G. Hardy
openaire   +3 more sources

The fractionation of histones by electrophoresis in polyacrylamide gel

Analytical Biochemistry, 1965
Abstract Increased interest in histone fractionation is evidenced by the many published electrophoretic techniques using starch (1–3) and polyacrylamide (4–6) gels. Twenty-two components of calf thymus histone have been identified by Neelin and Neelin (7) with starch gel electrophoresis. Driedger et al.
F.F. Davis, George F. Vande Woude
openaire   +3 more sources

Micro-Electrophoresis on Polyacrylamide Gels

1973
Polyacrylamide gels were introduced in 1959 by Raymond and Weintraub, as supports for electrophoretic separations. The polyacrylamide gel is produced by polymerising acrylamide, with N,N-methylenebisacrylamide or ethylene diacrylate as the cross-linking component. Catalytic redox systems, which yield free radicals, are used to initate copolymerisation (
openaire   +3 more sources

Polyacrylamide Gel Electrophoresis of Lysates of Mycoplasmas

Research in Veterinary Science, 1970
The technique of Razin & Rottem (1967) for electrophoretic comparison of mycoplasmas has been modified, principally by carrying out the electrophoresis on flat gel slabs, and has been used to examine lysates of several mycoplasma preparations. The 6 different species compared could readily be distinguished, while different batches and isolates of the ...
H. Zola, Lizbeth J. Sayer, W. Baxendale
openaire   +3 more sources

Polyacrylamide gel electrophoresis on micro slabs

Analytical Biochemistry, 1972
Abstract An electrophoretic technique using micro polyacrylamide flat gels is described and its usefulness demonstrated. The gels are vertically cast and electrophoresed in slab form (75 × 18 × 0.75 mm) in closed thin glass cells (cuvets) made from detachable microscope slides.
H. Rainer Maurer, Francesco A. Dati
openaire   +3 more sources

SDS Polyacrylamide Gel Electrophoresis of Proteins

2003
Probably the most widely used of techniques for analyzing mixtures of proteins is SDS polyacrylamide gel electrophoresis. In this technique, proteins are reacted with the anionic detergent, sodium dodecylsulfate (SDS, or sodium lauryl sulfate) to form negatively charged complexes.
openaire   +4 more sources