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ELISAs for parasitologists: or lies, damned lies and ELISAs
Parasitology Today, 1993The introduction in 1971, by Engvoll and Perlmann, of enzyme labels in immunoassays represented a significant technical advance. Their enzyme-linked immunosorbent assay (ELISA) proved to be as sensitive as radioimmunoossoys but safer to use. Since then, ELISAs have been widely used in the assay o f anti-bodies and antigens.
P, Venkatesan, D, Wakelin
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2003
Enzyme-linked immunosorbent assay (ELISA) is a very useful technique for the specific and sensitive assay of certain compounds, in which suitable antibodies, monoclonal or polyclonal, to the compounds are available. The technique has found particular application in the monitoring of environmental contaminants and toxins, either studying the primarily ...
K, Makarananda, G E, Neal
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Enzyme-linked immunosorbent assay (ELISA) is a very useful technique for the specific and sensitive assay of certain compounds, in which suitable antibodies, monoclonal or polyclonal, to the compounds are available. The technique has found particular application in the monitoring of environmental contaminants and toxins, either studying the primarily ...
K, Makarananda, G E, Neal
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2009
This chapter defines the terms and examines the configurations used for most applications of ELISA. Such a chapter is important because the possibilities inherent in the systems of ELISA must be understood in order to maximize their versatility in assay design. All heterogeneous systems have three basic parameters: 1.
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This chapter defines the terms and examines the configurations used for most applications of ELISA. Such a chapter is important because the possibilities inherent in the systems of ELISA must be understood in order to maximize their versatility in assay design. All heterogeneous systems have three basic parameters: 1.
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2015
The enzyme-linked immunosorbent assay (ELISA) is a simple and rapid technique for detecting and quantitating antibodies or antigens attached to a solid surface. Being one of the most sensitive immunoassays, ELISA offers commercial value in laboratory research, diagnostic of disease biomarkers, and quality control in various industries.
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The enzyme-linked immunosorbent assay (ELISA) is a simple and rapid technique for detecting and quantitating antibodies or antigens attached to a solid surface. Being one of the most sensitive immunoassays, ELISA offers commercial value in laboratory research, diagnostic of disease biomarkers, and quality control in various industries.
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2009
This chapter gives general information on essential practical features of ELISAs. These can be summarized as follows: 1. Adsorption of antigen or antibody to the plastic solid phase. 2. Addition of the test sample and subsequent reagents. 3. Incubation of reactants. 4. Separation of bound and free reactants by washing. 5.
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This chapter gives general information on essential practical features of ELISAs. These can be summarized as follows: 1. Adsorption of antigen or antibody to the plastic solid phase. 2. Addition of the test sample and subsequent reagents. 3. Incubation of reactants. 4. Separation of bound and free reactants by washing. 5.
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2015
First described by Engvall and Perlmann, the enzyme-linked immunosorbent assay (ELISA) is a rapid and sensitive method for detection and quantitation of an antigen using an enzyme-labeled antibody. Besides routine laboratory usage, ELISA has been utilized in medical field and food industry as diagnostic and quality control tools.
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First described by Engvall and Perlmann, the enzyme-linked immunosorbent assay (ELISA) is a rapid and sensitive method for detection and quantitation of an antigen using an enzyme-labeled antibody. Besides routine laboratory usage, ELISA has been utilized in medical field and food industry as diagnostic and quality control tools.
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Transactions of the Royal Society of Tropical Medicine and Hygiene, 1980
S, Mansueto, M D, Miceli
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S, Mansueto, M D, Miceli
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