Results 261 to 270 of about 137,065 (307)

A Simple Technique for Preparation of Chicken-Embryo-Skin Cell Cultures

Avian Diseases, 1982
A simple, rapid technique was developed for preparing chicken-embryo-skin cell cultures utilizing trypsinization of the skin of intact 12-day-old chicken embryos. When cell cultures were inoculated with fowl pox virus, those that consisted of at least 80% epithelial cells yielded a higher virus titer than fibroblast cell cultures.
A, Silim, M A, El Azhary, R S, Roy
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New serum‐free in vitro culture technique for midgestation mouse embryos

genesis, 2003
AbstractSummary: Current in vitro culture methods for mouse embryos are critically dependent on specially prepared rodent serum. Rodent serum requires careful preparation and stringent assessment of serum quality, while commercially available whole embryo culture serum is expensive and shows considerable lot variability.
Billie A, Moore-Scott, Julie, Gordon, C Clare, Blackburn, Brian G, Condie, Nancy R, Manley   +4 more
openaire   +2 more sources

Use of Desiccated Chick Embryo in Tissue Culture Technique

Nature, 1940
ONE of the difficulties inherent in tissue culture technique is the necessity for a regular supply of chick embryo extract, without which serial subcultures cannot be maintained. When fresh 9–11 day embryos are used, it is almost impossible to avoid a waste of material as it is necessary to incubate more eggs than will probably be required in order to ...
P. R. PEACOCK, R. I. SHUKOFF
openaire   +1 more source

Whole embryo culture: a “New” technique that enabled decades of mechanistic discoveries

Birth Defects Research Part B: Developmental and Reproductive Toxicology, 2010
AbstractDenis New's development of the rodent whole embryo culture (WEC) method in the early 1960s was a groundbreaking achievement that gave embryologists and teratologists an unprecedented degree of access to the developing postimplantation rodent embryo.
Robert G, Ellis-Hutchings, Edward W, Carney   +1 more
openaire   +2 more sources

Direct DNA delivery into zebrafish embryos employing tissue culture techniques

genesis, 2001
AbstractSummary: The production of transfected fish embryos requires expertise in injecting the fertilized eggs and/or expensive equipment for electroporation or microprojectiles. This article demonstrates that by exposure to DNA constructs conjugated with transfecting reagents dechorionated Danio rerio embryos are capable of acquiring extracellular ...
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A simple effective embryo culture technique for brassica

Euphytica, 1969
Sterilized ovules of a suitable age are cut in half and introduced to a culture medium kept in constant motion. Many of the embryos are washed out of the ovules and grow. The success rate compares well with more sophisticated techniques. Several plants of synthetic B. napus have been grown by this method.
openaire   +1 more source

A new technique for culturing rat embryos between gestation days 14 and 15

Toxicology in Vitro, 1993
A method for culturing rat embryos between days 14 and 15 has been developed. On gestation day 14 (morning of sperm and plug = day 0), laparotomies were performed on time-mated rats that had been anaesthetized with halothane. The embryos, with open attached yolk sacs and placentas, were cultured in 650-ml plastic flasks with 120 ml medium (0.7% bovine ...
C V, Barber, M B, Carda, A G, Fantel
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Microinjection of cultured rat embryos: A new technique for studies in chemical dysmorphogenesis

Toxicology and Applied Pharmacology, 1989
The utility of a new technique for exposure of cultured whole rat embryos to potential dysmorphogens was demonstrated with nitrosofluorene (NF), a cytotoxic and mutagenic metabolite of 2-acetylaminofluorene (AAF). At an initial concentration in the culture medium of 41 microM, NF produced a 100% incidence of defects in axial rotation with no ...
K L, Stark, M R, Juchau
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Explanted embryo culture: In vitro and in ovo techniques for domestic fowl

British Poultry Science, 1987
Abstract 1. Embryos of the domestic fowl (72 h old) have been explanted into shell‐less cultures or ‘surrogate’ eggshells, in order to investigate the possibility of rearing these embryos to hatching. 2. Rocking embryo cultures during the first half of incubation enhanced embryo growth. 3.
Karen Rowlett, K. Simkiss
openaire   +1 more source

Detection of virus infection in cowpea seeds through embryo culture technique

Ghana Journal of Science, 2009
No Abstract.
Gupta, MD, Rao, DR, Verma, VS
openaire   +2 more sources