Results 61 to 70 of about 137,065 (307)

Formation of Blastocysts From Zona Pellucida–Free Oocytes: A Case Report on a Modified Technique in In Vitro Fertilization

Case Reports in Obstetrics and Gynecology
Conclusions: By employing appropriate techniques for fertilization and embryo culture, ZFOs are capable of the development and production of good-quality blastocysts.
Minh Tam Le, Trung Van Nguyen, Hong Nhan Thi Dang, Quoc Huy Vu Nguyen   +3 more
doaj   +1 more source

Anchorage‐independent and faster growth in clonal population from UV‐irradiated NER‐deficient cells

FEBS Open Bio, EarlyView.
UV‐irradiated cells expressing a DDB2 mutant protein unable to interact with PCNA (DDB2PCNA‐) form clones able to grow without anchorage. Different experimental approaches reveal heterogeneity in cell cycle regulation and drug response within these clones, emphasizing the crucial role of the DDB2‐PCNA interaction in preventing cellular transformation ...
Paola Perucca, Anna Tricarico, Martina Furfaro, Priyam Ghosh, Ennio Prosperi, Lucia Anna Stivala, Ornella Cazzalini   +6 more
wiley   +1 more source

A technique for embryo culture of peaches

Hilgardia, 1950
This publication describes a technique. … for growing peach-embryo cultures. In laboratory experiments, a high percentage of the cultures was free of microorganisms that would develop under yeast-extract broth within 15 days at room temperature.
openaire   +2 more sources

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

A New Technique for the Sterile Culture of Plant Embryos in vitro [PDF]

Nature, 1952
THE two essentials for successful tissue culture are a sufficient supply of nutrients and adequate aeration. In the culture of plant embryos from an early stage of development it is also often desirable that cultures shall be suitable for microscopical examination.
openaire   +2 more sources

Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1

FEBS Open Bio, EarlyView.
The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer, Angelique Kragl, Katrin Ziems, Juliane Glaubitz, Sophie Grammbauer, Susanne Hildebrandt, Mladen V. Tzvetkov, Gabriele Jedlitschky   +7 more
wiley   +1 more source

Embryo culture and in vitro clonal multiplication of Prunus ‘Capdeboscq’ rootstock

Crop Breeding and Applied Biotechnology, 2003
Embryo culture and recovery are techniques used in basic and applied studies of morphogenesis, in vitro propagation and genetic breeding in the Prunus genus.
Aparecido Lima da Silva, Miguel Pedro Guerra, Marcelo Rogalski   +2 more
doaj  

Modelling aspects of oviduct fluid formation in vitro [PDF]

, 2016
© 2017 Society for Reproduction and Fertility. Oviduct fluid is the microenvironment that supports early reproductive processes including fertilisation, embryo cleavage and genome activation.
Arnold, Georg, Arnold, Georg J., Froehlich, Thomas, Fröhlich, Thomas, Leese, Henry, Leese, Henry J., Sathyapalan, Thozhukat, Simintiras, Constantine A., Sturmey, Roger, Sturmey, Roger G., Ulbrich, Susanne, Ulbrich, Susanne E.   +11 more
core   +2 more sources

Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs

FEBS Open Bio, EarlyView.
Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner, Hainan Zhu, Carol Christine Bosholm, Heuy‐Ching Wang, Tracy Criswell, Anthony Atala, Jian‐Xing Ma, Yuanyuan Zhang   +7 more
wiley   +1 more source

Analyzing Cell-free Genomic DNA in Spent Culture Media: Noninvasive Insight into the Blastocysts

Global Medical Genetics
A commonly accepted standard protocol for noninvasive techniques for the genetic evaluation of an embryo remains elusive due to inconclusiveness regarding the volume of spent media to be acquired and the possibility of acquiring the same for subsequent ...
Siddhartha Shankar Layek, Shrushti Kanani, Shilpa Doultani, Tejas Gohil, Sanket Patil, Ananthasayanam Sudhakar, Kathan Banubhai Raval, Karuppanasamy Kuppusamy, Sanjay Gorani, Sudharson Raj, Rafiya Sangameshwari, Himali Jadeja, Mini Mol P.   +12 more
doaj   +1 more source