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Plant Cell, Tissue and Organ Culture, 1993 The effects of four exogenous amino acids (proline, glycine, asparagine and serine) on the production of maize embryogenic callus and on its endogenous amino acid content have been investigated. For this purpose, an established embryogenic line of Type 1
I Claparols
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Embryogenic callus formation from maize protoplasts
Planta, 1987Maize (Zea mays L.) protoplasts have been obtained which divide rapidly and produce a callus that differentiates to form somatic embryos. The somatic embryos can be induced to form roots and small leaf-like structures. The genotype was the hybrid A188xBlack Mexican Sweet.
K K, Kamo +3 more
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Selection of embryogenic sugarcane callus by image analysis
Journal of Bioscience and Bioengineering, 1999In the cultivation of plant calli on solid media, two kinds of calli such as compact and friable calli, which are a bright yellow and a whitish clump, respectively, are often obtained. Distinction of these calli is of much importance in the regeneration step.
H, Honda +5 more
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Whisker-mediated transformation of embryogenic callus of maize
Plant Cell Reports, 2000The present study was designed to establish embryogenic callus as a target tissue for whisker-mediated transformation of maize (Zea mays L.). Silicon carbide whiskers were used to deliver the bar and uidA (GUS) genes into embryogenic maize callus. Samples of osmotically-treated Type II callus were vigorously agitated in the presence of whiskers and ...
J F, Petolino +3 more
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Arabinogalactan proteins in embryogenic and non-embryogenic callus cultures of Euphorbia pulcherrima
Physiologia Plantarum, 2000The arabinogalactan protein (AGP) fractions of embryogenic and non‐embryogenic callus lines of Euphorbia pulcherrima Willd. ex. Klotzsch were analysed over a cultivation period of 9 weeks using the β‐glucosyl Yariv reagent and an anti‐AGP antibody (LM2).
Katja Saare‐Surminski +3 more
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Isozymes as Markers for Embryogenic Maize Callus
1994The search for markers of plant embryogenesis is an important aspect of modern plant breeding and isozyme analysis remains a powerful tool to register cyto-differentiation in plants. The use of in situ cytochemical techniques to study the proteins which are expressed during embryogenesis has been underestimated so far. This chapter, mainly based on our
Schel, J.H.N. +2 more
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Marker proteins for embryogenic differentiation patterns in pea callus
Plant Cell Reports, 1987Polypeptide pattern alterations during somatic embryogenesis were investigated using callus cultures of two Pisum sativum genotypes. Both genotypes show the formation of two different callus lines from the same explant after six to eight weeks in culture: a nodular yellowish callus line, which forms somatic embryoids in suspension cultures (e(+)) and a
S, Stirn, H J, Jacobsen
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Cryopreservation of embryogenic callus ofArundo donaxL.
Acta Horticulturae, 2016Currently Arundo donax, a non-food perennial grass also known as giant reed, is getting increased attention due to its use in biofuel production and phytoremediation. The plant is traditionally propagated by rhizomes, stem nodes or embryo rescue, and none of these can fulfil today the increasing demand for its cultivation.
Ozudogru EA +4 more
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In Vitro Embryogenic Callus Formation in Chimonanthus
1988Capability of uninuclear microspores of the woody sp. Ch.praecox L. (2n=22) for androgenesis and/or callus production in the culture of anthers from the buds in successive developmental stages (1–4) and cultured in MS (Murashige and Skoog, 1962) medium with 2,4-D, Kin., and vitamines (Radojevic, 1980) was studied.
Lj. Radojević +2 more
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Development of a bioreactor suitable for embryogenic rice callus culture
Journal of Bioscience and Bioengineering, 1999Embryogenic rice calli induced from mature rice (Oryza sativa L., Sasanishiki) were cultured in a jarfermentor with disk turbine impellers, an air-lift reactor, and a turbine-blade reactor (TBR(R)), and the effects of agitation were investigated. In all cases, the growth inhibition was observed, though a slightly improved regeneration frequency was ...
K H, Moon, H, Honda, T, Kobayashi
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