Results 31 to 40 of about 38,507 (166)

Agrobacterium-mediated transformation of embryogenic callus and screening and validation of resistant callus.

open access: yes, 2021
A: Fresh embryogenic callus; B: Co-culture; C: Recovery culture; D, E, F: 1st, 2nd, and 3th screening culture.
Shanshan Yan (761415)   +7 more
core   +1 more source

Identification and comparative analysis of Identification and comparative analysis of microRNA in wheat (Triticum aestivum L.) callus derived from mature and immature embryos during in vitro culture

open access: yesFrontiers in Plant Science, 2016
Feasible and efficient tissue culture plays an important role in plant genetic engineering. Wheat (Triticum aestivum L.) immature embryos (IMEs) are preferred for tissue culture to mature embryos (MEs) because IMEs easily generate embryogenic callus ...
Chu Zong Li   +17 more
doaj   +1 more source

Uniconazole Augments Abscisic Acid in Promoting Somatic Embryogenesis in Cotton (Gossypium hirsutum L.)

open access: yesFrontiers in Plant Science, 2022
During somatic embryogenesis (SE), somatic cells initiate embryogenic development under appropriate conditions. Uniconazole, a plant growth regulator, was found to inhibit the proliferation of callus but promoted the conversion of callus into an ...
Yanli Chen   +9 more
doaj   +1 more source

Metabolic Rewiring of the Shikimate Pathway Enables Rose‐Scented Rice

open access: yesPlant Biotechnology Journal, EarlyView.
ABSTRACT Rice fragrance is a key determinant of grain quality, yet the aroma profile of cultivated rice is highly uniform and largely dominated by the popcorn‐like compound 2‐acetyl‐1‐pyrroline. Here we expanded the aromatic repertoire of rice by engineering a rose‐like fragrance through reconstruction of the phenylalanine‐derived 2‐phenylethanol (2‐PE)
Xiaoqing Cui   +11 more
wiley   +1 more source

Graft Biology in the CRISPR Era: From Tissue Fusion to Genome Compatibility

open access: yesPlant-Environment Interactions, Volume 7, Issue 5, October 2026.
ABSTRACT Plant lineage has traditionally constrained grafting compatibility, with monocots generally considered incompatible because of their dispersed vascular bundles and limited secondary growth. Recent studies have shown that embryonic grafting can establish successful graft unions in selected monocot systems by exploiting early developmental ...
Melagani Subash Chandra Gowda Sahaja   +5 more
wiley   +1 more source

Embriogênese somática em pupunheira (Bactris gasipaes), butiá-da-serra (Butia eriospatha) e açai (Euterpe oleracea): isolamento e cultura de protoplastos, avaliações bioquímicas e otimização das condições de cultura [PDF]

open access: yes, 2015
Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências Agrárias, Programa de Pós-Graduação em Recursos Genéticos Vegetais, Florianópolis, 2015.A embriogênese somática (ES) é um métedo eficiente à propagaçãomassiva de plantas ...
Ree, Joseph Francis
core  

Embryogenic Callus Formation in Local Cocoa (Theobroma Cacao L.) Clones [PDF]

open access: yes, 2006
A study on the potential of embryogenic callus formation in KKM 1,KKM 15, KKM 17, KKM 22, KKM 27, KKM 28, PBC 123, PBC 159, MHP 78,MHP 79, MHP 296, AMAZ, GS 29, EET 339 and MJS 47 local cocoa clones which had been released by MARDI was carried out.
Ramli, Asfaliza
core   +1 more source

The combination of morphogenic regulators BABY BOOM and GRF‐GIF improves maize transformation efficiency and promotes leaf regeneration

open access: yesNew Phytologist, Volume 252, Issue 1, Page 260-275, October 2026.
Maize regeneration via somatic embryogenesis by ectopic expression of morphogenic factors TaGRF4‐TaGIF1 and ZmBBM. Summary Transformation is an indispensable tool for plant genetics and functional genomics. Although stable transformation in maize is no longer a major obstacle, there remains a need for accessible and efficient methods for academic ...
Zongliang Chen   +8 more
wiley   +1 more source

A Robust Framework for Maize Elite Line Genome Editing Through Enhanced HI‐Edit via LbCas12a Activity Optimization

open access: yesPlant Biotechnology Journal, Volume 24, Issue 10, Page 5392-5404, October 2026.
ABSTRACT Haploid induction coupled with genome editing (HI‐Edit) enables direct modification of commercial crop varieties, bypassing the need for trait introgression or direct transformation of elite lines with CRISPR machinery. However, its widespread application has been constrained by low haploid editing rates (HER), the proportion of haploids ...
Dawei Liang   +21 more
wiley   +1 more source

Targeted Modulation of Eukaryotic Release Factor 1 (OseRF1) by an RNA Aptamer Enhances Drought Adaptation in Rice

open access: yesPlant Biotechnology Journal, Volume 24, Issue 10, Page 5440-5459, October 2026.
ABSTRACT Drought stress severely restricts rice productivity, necessitating strategies to enhance stress resilience. Here, we identify translation termination as a tunable regulatory node for stress adaptation. Using 25 rounds of SELEX, we isolated RTAR, an RNA aptamer that binds the rice eukaryotic release factor 1 (OseRF1) with nanomolar affinity ...
Shuangfeng Dai   +7 more
wiley   +1 more source

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