Results 251 to 260 of about 928,279 (312)

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Semitendinosus Autograft Reconstruction for Neglected Patellar Tendon Rupture: Technical Simplicity with Excellent Outcome. [PDF]

J Orthop Case Rep
Raghute S, Dwidmuthe S, Das D, Surushe A, Chandrakar D, Priyanshu.   +5 more
europepmc   +1 more source

Time‐restricted feeding prior to Mycobacterium tuberculosis infection reduces tissue CD4+ T cells with limited impact on bacterial clearance

FEBS Open Bio, EarlyView.
Time‐restricted feeding (TRF) in mice increased liver fatty acid oxidation and decreased fatty acid biosynthesis. These alterations persisted when TRF was discontinued and the host was infected with Mycobacterium tuberculosis. Pre‐exposure to TRF did not alter tissue (lung and spleen) mycobacterial burden but significantly reduced CD3+ T cells in lungs
Ashish Gupta, Nidhi Yadav, Subhasmita Das, R. Rajendra Kumar Reddy, Nupur Sharma, Amol Ratnakar Suryawanshi, Jaswinder Singh Maras, Ranjan Kumar Nanda   +7 more
wiley   +1 more source

Microtubule end stabilisation by cooperative oligomers of Ska and Ndc80 complexes. [PDF]

EMBO J
Radhakrishnan RM, Stokes L, Day M, Huis In 't Veld PJ, Volkov VA.   +4 more
europepmc   +1 more source

Hydrostatic pressure activates HIF‐1α via β‐catenin to promote stemness in breast cancer cells

FEBS Open Bio, EarlyView.
To mimic the elevated intestinal fluid pressure in breast cancers, we loaded human breast cancer cells (MCF‐7, MDA‐MB‐453, and BT‐474) to 50 mmHg hydrostatic pressure. Hydrostatic pressure exposure upregulated HIF‐1α and induced stemness in MCF‐7 and BT‐474 cells.
Da Zhai, Yong Xu, Jingjing Ma, Ratchanee Duangrat, Chen Yan, Jingyan Liang, Hua Wei, Bin Jiang, Tao‐Sheng Li   +8 more
wiley   +1 more source

Groups acting on trees with Tits' independence property (P): With an appendix by Stephan Tornier. [PDF]

Math Ann
Reid CD, Smith SM.
europepmc   +1 more source

Noncanonical chromosomal-end-specific telomeric repeat arrays in naturally telomerase-negative yeasts. [PDF]

Nucleic Acids Res
Brejová B, Hodorová V, Lichancová H, Gafurov A, Bujna D, Brázdovič F, Červenák F, Petrík T, Hegedűsová E, Forgáčová Jakúbková M, Neboháčová M, Tomáška Ľ, Sipiczki M, Vinař T, Nosek J.   +14 more
europepmc   +1 more source

Generation of precise and accurate engineered circRNAs using enzymatic ligation. [PDF]

Nucleic Acids Res
Singh A, Dujsikova A, Mueller N, Chen YG.   +3 more
europepmc   +1 more source

Cigarette smoking abstinence at follow-up at 12 months among US adults who regularly used Electronic Nicotine Delivery Systems and smoked in the past year: A prospective cohort study. [PDF]

Tob Induc Dis
Nyman AL, Henderson KC, Ashley DL, Spears CA, Huang J, Duan Z, Weaver SR.   +6 more
europepmc   +1 more source