Results 91 to 100 of about 7,300,385 (295)
FEBS Open Bio, EarlyView.Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Zero energy homes: Combining energy efficiency and solar energy technologies
, 2000 In a typical Florida house, air-conditioning accounts for about 35% of all the electricity the home uses. As the largest single source of energy consumption in Florida, a home's air-conditioning load represents the biggest energy challenge facing Florida
National Renewable Energy Laboratory (U.S.)
core
FEBS Open Bio, EarlyView.The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong +3 more
wiley +1 more source
FEBS Open Bio, EarlyView.Lactoferrin promotes wound healing by activating MAPK, PI3K/Akt, and extracellular matrix pathways, enhancing fibroblast remodeling, proliferation, and re‐epithelialization. These mechanisms highlight its therapeutic potential in inflammation control, tissue repair, and remodeling.
Morgana Lüdtke Azevedo +4 more
wiley +1 more source
Energy Flow of Watermelon Production System for Optimizing Energy Efficiency [PDF]
Iranica Journal of Energy and EnvironmentThis research, conducted in Gotvand, southwest Iran, evaluated the energy balance of a field system which watermelon produced in it. In the current research, energy inputs of watermelon planting were measured.
S. N. Mosavian, H. Eskandari
doaj +1 more source
FEBS Open Bio, EarlyView.Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty +13 more
wiley +1 more source
140–400-mV Input Switched-Capacitor Converter for Miniaturized Photovoltaic Energy Harvesting
IEEE AccessThis article presents a wide operating range switched-capacitor (SC) converter designed for miniaturized photovoltaic (PV) energy sources. In millimeter-scale devices, PV power transfer is limited by low generated voltage and available power, posing ...
Reyhan Ramadhan +3 more
doaj +1 more source
Energy balance for carrot root production and drying [PDF]
Journal on Processing and Energy in Agriculture, 2014 Energy balance for field production and drying of carrot root, that was sowed in spring, was done in 2012. Carrot root was grown in a field in Despotovo village and the produced yield was 69,000 kg/ha.
Bajkin Anđelko +4 more
doaj