Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Analysing the Effect of Energy Intensity on Carbon Emission Reduction in Beijing. [PDF]
Int J Environ Res Public Health, 2023 Li G, Zeng S, Li T, Peng Q, Irfan M.
europepmc +1 more source
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Inequality across countries in energy intensities: an analysis of the role of energy transformation and final energy consumption [PDF]
This paper analyzes the role of the energy transformation index and of final energy consumption per GDP unit in the disparities in energy intensity across countries.
Emilio Padilla, Juan Antonio Duro
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Impact of financial development on the energy intensity of developing countries. [PDF]
Heliyon, 2022 Ma Y, Zhao Y, Jia R, Wang W, Zhang B.
europepmc +1 more source
Versatile vector tools for efficient protein screening across multiple expression systems
FEBS Open Bio, EarlyView.A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
Energy Conservation in the United States: Understanding its Role in Climate Policy [PDF]
Efforts to reduce carbon emissions significantly will require considerable improvements in energy intensity, the ratio of energy consumption to economic activity. Improvements in energy intensity over the past thirty years suggest great possibilities for
Gilbert E. Metcalf
core
Magnitude and determinants of energy intensity: evidence from Indian firms. [PDF]
Environ Sci Pollut Res Int, 2023 Kumar A, Mittal A, Pradhan AK.
europepmc +1 more source
Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids
FEBS Open Bio, EarlyView.Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão +3 more
wiley +1 more source
A study of changing patterns of energy consumption and energy efficiency in the Indian manufacturing sector [PDF]
In the present economic scenario, the key concerns in policy making in India are: how to achieve higher energy efficiency in the key sectors and how to reduce the vulnerability of the economy to external shocks. In this paper, an attempt is made to study
Jena, Pradyot
core