Results 71 to 80 of about 4,234,556 (345)
Structural insights into an engineered feruloyl esterase with improved MHET degrading properties
FEBS Letters, EarlyView.A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa +5 more
wiley +1 more source
Youqicang pingjia yu kaifa, 2021 During drilling the horizontal section of a shale gas well, oil-based drilling fluid is commonly used in China in order to ensure downhole safety, especially to solve the problems of lost circulation and well collapse.
CHEN Liang, HU Jinke, GENG Dong, LI Ziyu
doaj
OntoEng: A design method for ontology engineering in information systems [PDF]
, 2009 This paper addresses the design problem relating to ontology engineering in the discipline of information systems. Ontology engineering is a realm that covers issues related to ontology development and use throughout its life span.
Al-Debei, MM, Fitzgerald, G
core
FEBS Letters, EarlyView.This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel +13 more
wiley +1 more source
FEBS Letters, EarlyView.Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura +2 more
wiley +1 more source
Application of Polymer Curing Agent in Ecological Protection Engineering of Weak Rock Slopes
Applied Sciences, 2019 Under the action of water, weak rock slopes easily expand and the strength and stiffness decrease, which results in slope instability. The styrene–acrylic emulsion cement-matrix composite, a new type of polymer curing agent, was developed for the ...
Ding Yao +3 more
doaj +1 more source
Towards a methodology for rigorous development of generic requirements patterns
, 2005 We present work in progress on a methodology for the engineering, validation and verification of generic requirements using domain engineering and formal methods.
Johnson, Ian +2 more
core
The Concept of Integrity and Its Application to Engineering Ethics [PDF]
, 2014 Integrity is a common and well established concept in the ethical vocabulary. It is also being used in various codes of ethics, and investigated by means of various methods.
Wajszczyk, Piotr
core
Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation
FEBS Letters, EarlyView.Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz +11 more
wiley +1 more source
Aggressive prostate cancer is associated with pericyte dysfunction
Molecular Oncology, EarlyView.Tumor‐produced TGF‐β drives pericyte dysfunction in prostate cancer. This dysfunction is characterized by downregulation of some canonical pericyte markers (i.e., DES, CSPG4, and ACTA2) while maintaining the expression of others (i.e., PDGFRB, NOTCH3, and RGS5).
Anabel Martinez‐Romero +11 more
wiley +1 more source