Results 171 to 180 of about 754,142 (199)

Rapid generation of prion disease models using AAV‐delivered PrP variants in knockout mice

Brain Pathology, EarlyView.
We developed a rapid AAV‐based system to generate prion disease models in weeks rather than months. Following systemic AAV9P31 delivery of modified PrP to knockout mice, we achieved brain‐wide expression and successful propagation of both classical (RML) and atypical (GSS‐A117V) prion strains.
Maitena San‐Juan‐Ansoleaga, Eva Fernández‐Muñoz, Jorge M. Charco, Enric Vidal, Diego Herrero‐Martínez, Josu Galarza‐Ahumada, Cristina Sampedro‐Torres‐Quevedo, Samanta Giler, Mariví Geijo, Gloria González‐Aseguinolaza, Hasier Eraña, Joaquín Castilla   +11 more
wiley   +1 more source

The L108I polymorphism in mouse prion protein drives spontaneous disease and enhances transmission of atypical and classical prion strains

Brain Pathology, EarlyView.
A single amino acid change (L108I) combined with PrP overexpression drives spontaneous atypical prion formation in mice, enabling also efficient propagation of diverse prion strains. This model allows studying how spontaneous prion diseases arise and provides powerful tools for investigating strain emergence, transmission barriers, and mechanisms ...
Hasier Eraña, Enric Vidal, Natalia Fernández‐Borges, Jorge M. Charco, Carlos M. Díaz‐Domínguez, Cristina Sampedro‐Torres‐Quevedo, Josu Galarza‐Ahumada, Eva Fernández‐Muñoz, Maitena San‐Juan‐Ansoleaga, Miguel Ángel Pérez‐Castro, Nuno Gonçalves‐Anjo, Patricia Piñeiro, Samanta Giler, Nora González‐Martín, Nuria L. Lorenzo, Africa Manero‐Azua, Guiomar Perez de Nanclares, Mariví Geijo, Manuel A. Sánchez‐Martín, Jesús R. Requena, Joaquín Castilla   +20 more
wiley   +1 more source

Divergence in μ and δ opioid receptor pharmacology in neurons and antinociceptive efficacy in neuropathic pain: Insights from MP135 and CYM51010

British Journal of Pharmacology, EarlyView.
Background and purpose Opioids targeting the μ opioid receptor remain largely ineffective in neuropathic pain conditions, emphasizing the need for novel therapeutic strategies. MP135 was reported to reduce thermal nociception in naive animals by activating μ‐δ opioid receptor heteromers.
Perrine Inquimbert, Chantal Fitterer, Sylvain Hugel, Mila Jesic, Yannick Goumon, Virgine Andry, Severine Schneider, Francois Daubeuf, Abdelfattah Faouzi, Stephane Doridot, Susruta Majumdar, Frederic Bihel, Martine Schmitt, Dominique Massotte   +13 more
wiley   +1 more source

Enhancing antitumour response to proteasome inhibitors with inhibitors of insulin‐degrading enzyme, a new molecular vulnerability in multiple myeloma

British Journal of Pharmacology, EarlyView.
Inhibitors of insulin‐degrading enzyme boost PI cytotoxicity through an increased sensitivity of proteasome to PI inhibitors, induction of ISR, DNA damage and Myc down‐regulation. They overcome PI resistance in vitro and induce tumour regression in vivo.
Laetitia Lesire, Rebecca Deprez‐Poulain, Damien Bosc, Florence Leroux, Valerie Landry, Sarah Ourabah, Morgane Tardy, Clara Maillard, Ronan Gealageas, Julie Dumont, Adrien Herledan, Sandrine Warenghem, Manon Cruette, Celine Lenglart, Alexandre Biela, Catherine Piveteau, Eva De Smedt, Catharina Muylaert, Wenguang Liang, Elvira Garcia De Paco, Elina Alaterre, Sara Ovejero, Nicolas Robert, Fabien Delahaye, Wei‐Jen Tang, Peter van Endert, Elke De Bruyne, Jérôme Moreaux, Benoit Deprez   +28 more
wiley   +1 more source

Vergleich eines 3D‐gedruckten Hautmodells mit etablierten Methoden zur Lehre der Stanzbiopsie mit Nahtversorgung

JDDG: Journal der Deutschen Dermatologischen Gesellschaft, EarlyView.
Zusammenfassung Hintergrund Realistische Trainingsmodelle sind essenziell, um Ärztinnen und Ärzte in klinischen Prozeduren zu schulen. In dieser Studie wurde untersucht, ob ein kostengünstiges, selbst generiertes 3D‐gedrucktes Hautmodell Früchten und Schaumstoffmodellen beim Erlernen von Hautstanzen überlegen ist.
Sandra Schuh, Stefan Schiele, Anna Rubeck, Ludwig Christian Hinske, Julia Welzel, Alexander Schneller   +5 more
wiley   +1 more source

Born to run? Associations between gestational and early‐life exposures and later‐life performance outcomes in Thoroughbreds

Equine Veterinary Journal, EarlyView.
Abstract Background Gestational and early‐life exposures may modulate development during growth and influence future athletic performance. Objectives To investigate associations between gestational and early‐life exposures in Thoroughbreds and (i) likelihood of racing, (ii) total number of runs and (iii) total prizemoney by the end of the 3‐year‐old ...
Rebecca Mouncey, Amanda M. de Mestre, Juan Carlos Arango‐Sabogal, Kristien L. Verheyen   +3 more
wiley   +1 more source

How quality improvement in health care can help to achieve the Millennium Development Goals

Bulletin of the World Health Organization
Anselm Schneider
doaj   +1 more source

An Angler‐Friendly AI Pipeline for Self‐Reporting and Automatic Catch Analysis in Recreational Fisheries

Fish and Fisheries, EarlyView.
ABSTRACT Monitoring recreational fisheries is difficult: anglers are widely dispersed, gear and practices vary, and many species are involved, which leads to fragmented and scarce data. To address these issues, we developed an Artificial Intelligence (AI) pipeline that turns angler‐reported photos into standardised records of catch composition and ...
Marco Signaroli, Bernat Morro, Eugenio Cutolo, Ignacio A. Catalán, Inmaculada Riera, Antoni Mira, Clara Mecinas, Antoni M. Grau, Yolanda Gonzalez‐Cid, Josep Alós   +9 more
wiley   +1 more source

Moderate increase of MET in hepatocytes protects against cholestatic liver injury by promoting an effective antioxidant response

The FEBS Journal, EarlyView.
Moderate overexpression of wild‐type Met in hepatocytes (Alb‐R26Met mouse model) boosts a strong antioxidant response dependent on the glutathione system while impairing TGF‐β signaling in the liver. This leads to improved liver regeneration and protects against DDC‐induced injury, a model for cholestatic disease sharing features with primary ...
Carlos González‐Corralejo, Juan García‐Sáez, María Figueroa‐Fuentes, Annalisa Addante, Cesáreo Roncero, Nerea Lazcanoiturburu, Beatriz Pacheco, Julián Sanz, Cristina Gato, Eva Fernández‐Calderón, María de la O López, Águeda González‐Rodríguez, Almudena Porras, Flavio Maina, Isabel Fabregat, Blanca Herrera, Aránzazu Sánchez   +16 more
wiley   +1 more source

Selective targeting of cortactin tandem repeat acetylation by human lysine deacetylases

The FEBS Journal, EarlyView.
Cortactin function is regulated by acetylation at several lysine residues within its tandem repeat region. Using genetic code expansion to generate cortactin variants containing precisely defined acetylation marks, we show that HDAC6 is the primary enzyme removing these modifications, with SIRT1 and SIRT2 also acting at selected sites but with lower ...
Jan Komarek, Miroslava Vosahlikova, Zsofia Kutil, Zora Novakova, Julia Kudlacova, Ruzena Tuckova, Marat Meleshin, Barbora Havlinova, Pavlina Jaklova, Jana Ptackova, Cordelia Schiene‐Fischer, Mike Schutkowski, Cyril Barinka   +12 more
wiley   +1 more source