Results 191 to 200 of about 1,506,434 (336)

Moving environmental DNA (eDNA) technologies from benchtop to the field using passive sampling and PDQeX extraction [PDF]

, 2022
Gert‐Jan Jeunen, Ulla von Ammon, Hugh Cross, Sara Ferreira, Miles D. Lamare, Robert C. Day, Jackson Treece, Xavier Pochon, Anastasija Zaiko, Neil J. Gemmell, Jo‐Ann L. Stanton   +10 more
openalex   +1 more source

Evolutionary dynamics of the chloroplast genome in Daphne (Thymelaeaceae): comparative analysis with related genera and insights into phylogenetics

FEBS Open Bio, EarlyView.
Comparative analysis of chloroplast genomes from 14 genera of Thymelaeaceae revealed variation in gene content, ranging from 128 to 142 genes, primarily influenced by IR expansion/contraction events and pseudogenization of ndhF, ndhI, and ndhG. Two large inversions were detected within the large single‐copy region, including a synapomorphic inversion ...
Abdullah, Hui Li, Rushan Yan, Chengyu Chen, Madiha Islam, Abdul Majid, Ibrar Ahmed, Parviz Heidari, Xiaoxuan Tian   +8 more
wiley   +1 more source

Detection of the elusive Dwarf sperm whale (Kogia sima) using environmental DNA at Malpelo island (Eastern Pacific, Colombia) [PDF]

, 2021
Jean‐Baptiste Juhel, Virginie Marques, Andrea Polanco F., Giomar Helena Borrero‐Pérez, Maria Mutis Martinezguerra, Alice Valentini, Tony Déjean, Stéphanie Manel, Nicolas Loiseau, Laure Velez, Régis Hocdé, Tom B. Letessier, Eilísh Richards, Florine Hadjadj, Sandra Bessudo, Felipe Ladino, Camille Albouy, David Mouillot, Loïc Pellissier   +18 more
openalex   +1 more source

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

FEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq, Nestor G. Casillas‐Vega, Alma Gomez‐Loredo, Xristo Zarate   +3 more
wiley   +1 more source

Autophagosome marker, LC3, is released extracellularly via several distinct pathways

FEBS Open Bio, EarlyView.
This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito, Masashi Arakawa, Koki Maeda, Eiji Morita   +3 more
wiley   +1 more source

pH-Dependent Degradation of Macrobial Environmental DNA in Water. [PDF]

Mol Ecol Resour
Jo TS.
europepmc   +1 more source

Establishment of a coculture system for Porphyromonas gingivalis and head and neck squamous cell carcinoma using spheroid culture and LATS inhibition

FEBS Open Bio, EarlyView.
We established a spheroid coculture system enabling viable Porphyromonas gingivalis–HNSCC interactions under normoxic conditions. Inhibition of LATS1/2 maintains tumor cells in an undifferentiated state, which may promote spheroid growth and create a more permissive environment for bacterial persistence.
Yurika Nakajima, Shogo Okazaki, Muneaki Tamura, Shuichi Sato, Kenichi Imai   +4 more
wiley   +1 more source

Advancing schistosomiasis monitoring through optimised environmental DNA detection. [PDF]

Food Waterborne Parasitol
Wambui CW, Viaene M, Mwangi HN, André B, Oguttu DW, Tolo CU, Hellemans B, Huyse T, Gante HF.   +8 more
europepmc   +1 more source

Antibiofilm activity of a chionodracine‐derived peptide by NMR‐based metabolomics of cell‐free supernatant of Acinetobacter baumannii clinical strains

FEBS Open Bio, EarlyView.
KHS‐Cnd peptide is able to impair biofilm formation and disaggregate mature biofilms in Acinetobacter baumannii clinical isolates. Differences in extracellular metabolites reflect changes in biofilm metabolism due to KHS‐Cnd treatment. Among the differentially represented extracellular metabolites upon KHS‐Cnd treatment, the significantly altered ...
Fernando Porcelli, Enrico Landi, Francesco Maiurano, Irene Paris, Rosanna Papa, Marco Artini, Laura Selan, Stefano Borocci, Francesco Buonocore, Esther Imperlini   +9 more
wiley   +1 more source

Environmental DNA (eDNA) Technology in Biodiversity and Ecosystem Health Research: Advances and Prospects. [PDF]

Ecol Evol
Wu S, Wang Y, Qin H, Zhang Z, Liu S, Ruan Y, Chen G, Yuan X, Zhang H.   +8 more
europepmc   +1 more source