Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Internet usage and environmental governance satisfaction in China: environmental pollution perception as a mediator. [PDF]
Huang X.
europepmc +1 more source
Is the EU shirking responsibility for its deforestation footprint in tropical countries? Power, material, and epistemic inequalities in the EU's global environmental governance. [PDF]
Kumeh EM, Ramcilovic-Suominen S.
europepmc +1 more source
Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima +7 more
wiley +1 more source
Challenges in assessing the effects of environmental governance systems on conservation outcomes. [PDF]
Ayambire RA +17 more
europepmc +1 more source
Corporate environmental governance scheme and investment efficiency over the course of COVID-19. [PDF]
Liu H, Jiang J, Xue R, Meng X, Hu S.
europepmc +1 more source
Research is strongest when conducted alongside patients, not just about them. Patient research organizations help integrate patient perspectives into research priorities, study design, and scientific meetings, leading to meaningful patient outcomes and development of relevant therapies.
Jenica H. Kakadia +9 more
wiley +1 more source
Government environmental governance, fiscal decentralization, and carbon intensity of the construction industry. [PDF]
Sun Y, Liu M, Lv Y.
europepmc +1 more source
A minimal cellulosome‐like system in Cellulosilyticum lentocellum
Cellulose‐degrading bacteria typically use cellulosomes, large multi‐enzyme complexes on a scaffold protein. In Cellulosilyticum lentocellum, we characterise a far smaller arrangement, a single scaffold bound to one cellulase through a single cohesin‐dockerin interaction.
John Allan +2 more
wiley +1 more source
Intellectual capital, digital transformation and firms' financial performance: Evidence from ecological protection and environmental governance industry in China. [PDF]
Yin J, Xu J.
europepmc +1 more source

