Results 221 to 230 of about 791,475 (315)

Resources consumption and environmental impacts of the DYNAMIC digital health intervention aimed at improving quality of care for sick children in Tanzania: a life cycle assessment. [PDF]

Front Digit Health
Karlen M, Emery N, Tan R, Kavishe G, Agrea P, Renggli S, Kulinkina AV, Luwanda L, Isdory G, Mtebene I, Mangu C, Castella PS, Bengoa X, D'Acremont V.   +13 more
europepmc   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Replacing meat and dairy with plant-based alternatives in the Netherlands: trade-offs in environmental impacts and critical nutrient intake. [PDF]

Eur J Nutr
Zhu Y, Politiek ACL, de Vet E, Ocké MC.
europepmc   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Assessing the Nutritional and Environmental Impacts of Major UK Food and Beverage Manufacturers

Hammond E, Bandy LK, Milner-Gulland E, Pechey R, Rayner M, Scarborough P, Jain S, Clark M.   +7 more
europepmc   +1 more source

Livestock Integration Into Cropping Systems Enhances Their Climate Change Resistance and Mitigation While Reducing Their Environmental Impacts. [PDF]

Glob Chang Biol
Delandmeter M, Basso B, Fettweis X, Lacroix C, Aubry P, Bindelle J, Dumont B.   +6 more
europepmc   +1 more source

Heterotropic regulation and negative homotropic cooperativity

FEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea, Francesco Angelucci, Federica Arnesano, Gianmarco Pascarella, Patrizio Di Micco, Andrea Bellelli   +5 more
wiley   +1 more source

Deriving breeding goals and expected selection responses to reduce environmental impacts in rainbow trout farming. [PDF]

Genet Sel Evol
Pouil S, Aubin J, Phocas F.
europepmc   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source