Results 101 to 110 of about 2,493,169 (277)

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Developing an Integrated Monitoring and Evaluation Flow for Sustainable Investment Projects [PDF]

Under the circumstances of certain weaknesses in the monitoring and evaluation processes of sustainable investment projects, the paper aims to develop a general integrated flow, encompassing both a project monitoring system and also a project evaluation ...
Florin TACHE
core  

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

Research Progress, Potential Environmental Impacts in Marine Geoengineering and Management Recommendations in China

Haiyang Kaifa yu guanli
Marine geoengineering has garnered considerable attention as a promising avenue for climate change mitigation. Within its two principal branches, research on marine carbon dioxide removal (CDR) has demonstrated substantial progress as demonstrated by a ...
WEI Shuyun, YANG Zhengxian, HAN Jianbo, XU Xuemei, WANG Xiaomeng, SU Qing   +5 more
doaj  

If services aren't delivered, people won't pay: the role of measurement problems and monitoring in Payments for Environmental Services [PDF]

The idea of Payments for environmental services (PES) has an appealing simplicity, which may explain the success of the concept. However, successful projects are far limited though and two constraints have been identified in literature.
Meijerink, Gerdien W.
core   +1 more source

Eighteenth Year of the Gulf of Maine Environmental Monitoring Program [PDF]

, 2009
This report summarizes the metals and organic contaminant data associated with the collection and analyses of blue mussel (Mytilus edulis) tissue from selected sites along the Gulf of Maine coast during the 2008 sampling season. Contaminant monitoring is
Aube, Jamie, Brun, Guy, Harding, Gareth, Hennigar, Peter, Jones, Stephen H., Krahforst, Christian, LeBlanc, Lawrence A., Page, David, Roach, Shawn, Schwartz, Jack, Shaw, Susan, Stahlnecker, James, Taylor, Darrell, Thorpe, Bruce, Wells, Peter   +14 more
core   +1 more source

Decreased cold‐sensing function of the transient receptor potential channel TRPM8 from tailed amphibians

FEBS Open Bio, EarlyView.
Despite frogs avoiding low temperatures, examination of four salamander species revealed that none avoided cold and all possessed cold tolerance. Functional analysis of TRPM8, a cold sensor, showed that all salamander TRPM8s had lost their cold sensitivity.
Tadahiro Sawao, Daiki Kobayashi, Shogo Hori, Osamu Saitoh   +3 more
wiley   +1 more source

Research on Collaborative Supervision Technology for Sea Sand Mining Under the Concept of Ecological Environment Protection

Haiyang Kaifa yu guanli
During the 14th Five-Year Plan period, China’s marine ecological environment protection still faces multiple challenges. Especially under the current rapid economic development, the rapid growth of infrastructure construction has led to abundant sea sand
LIU Ke, WANG Xiaomeng, YU Yue, WANG Xinxin, SUN Ruijun, HAN Jianbo   +5 more
doaj  

Digital twins to accelerate target identification and drug development for immune‐mediated disorders

FEBS Open Bio, EarlyView.
Digital twins integrate patient‐derived molecular and clinical data into personalised computational models that simulate disease mechanisms. They enable rapid identification and validation of therapeutic targets, prediction of drug responses, and prioritisation of candidate interventions.
Anna Niarakis, Philippe Moingeon
wiley   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source