Results 241 to 250 of about 1,477,177 (315)

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

Open-Source System Suitability: Mass Spectrometry Query Language Lab (MassQLab). [PDF]

Rapid Commun Mass Spectrom
Winter HL, Johnson D, Jarmusch AK.
europepmc   +1 more source

Thrombolytic proteins profiling: High‐throughput activity, selectivity, and resistance assays

FEBS Open Bio, EarlyView.
We present optimized biochemical protocols for evaluating thrombolytic proteins, enabling rapid and robust screening of enzymatic activity, inhibition resistance, and fibrin affinity, stimulation, and selectivity. The outcome translates to key clinical indicators such as biological half‐life and bleeding risk. These assays streamline the development of
Martin Toul, Alan Strunga, Jiri Damborsky, Zbynek Prokop   +3 more
wiley   +1 more source

Protocol for development of SPIRIT and CONSORT extensions for reporting climate and environmental outcomes in randomised trials (SPIRIT-ICE and CONSORT-ICE). [PDF]

BMJ Open
Petersen JJ, Hemberg L, Thabane L, Hopewell S, Chan AW, Hróbjartsson A, Mathiesen O, Kandasamy S, Siegfried N, Williamson PR, Fox L, Kamp CB, Hoffmann JM, Brorson S, Boutron I, McGain F, McAlister S, Mutengu L, Slutzman J, Sherman JD, Bentzer P, Jakobsen JC.   +21 more
europepmc   +1 more source

Exam‐level analysis of lecture capture viewing and student exam performance

FEBS Open Bio, EarlyView.
This study asks how lecture capture relates to exam performance in biology courses with three noncumulative exams. Mixed‐effects modeling separated between‐students from within‐student effects on exam %. Students who watched more recordings earned higher grades, but viewing spikes above a student's own mean, consistent with cramming, predicted small ...
Kirk Hillsley
wiley   +1 more source

Reporting of environmental outcomes in randomised clinical trials: a protocol for a scoping review. [PDF]

BMJ Open
Petersen JJ, Hemberg L, Thabane L, Hopewell S, Chan AW, Hróbjartsson A, Mathiesen O, Kandasamy S, Siegfried N, Williamson PR, Fox L, Kamp CB, Hoffmann JM, Brorson S, Jakobsen JC, Bentzer P.   +15 more
europepmc   +1 more source

Overview of molecular signatures of senescence and associated resources: pros and cons

FEBS Open Bio, EarlyView.
Cells can enter a stress response state termed cellular senescence that is involved in various diseases and aging. Detecting these cells is challenging due to the lack of universal biomarkers. This review presents the current state of senescence identification, from biomarkers to molecular signatures, compares tools and approaches, and highlights ...
Orestis A. Ntintas, Sylvia Vagena, Pavlos Pantelis, Giorgos Theocharous, Russel Petty, Konstantinos Evangelou, Vassilis G. Gorgoulis   +6 more
wiley   +1 more source

Author Correction: Carbon storage through China's planted forest expansion. [PDF]

Nat Commun
Cheng K, Yang H, Tao S, Su Y, Guan H, Ren Y, Hu T, Li W, Xu G, Chen M, Lu X, Yang Z, Tang Y, Ma K, Fang J, Guo Q.   +15 more
europepmc   +1 more source

Molecular determinants of signal transduction in tropomyosin receptor kinases

FEBS Open Bio, EarlyView.
Tropomyosin receptor kinases control critical neuronal functions, but how do the same receptors produce diverse cellular responses? This review explores the structural mechanisms behind Trk signaling diversity, focusing on allosteric modulation and ligand bias.
Giray Enkavi
wiley   +1 more source

Does China's corporate two-way foreign direct investment mitigate environmental pollution? [PDF]

PLoS One
Yang F, Chen T, Han L, Wang S.
europepmc   +1 more source