Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty +13 more
wiley +1 more source
Reply to Li et al.: Fluorinated liquid crystal monomers highlight a need for more data on emerging PFAS. [PDF]
Sun JM, Sunderland EM.
europepmc +1 more source
In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton +6 more
wiley +1 more source
Probabilistically defining environmentally relevant concentrations in ecotoxicology. [PDF]
Sun T +5 more
europepmc +1 more source
Adenosine triphosphate as a modulator of protein interactions and stability
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley +1 more source
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Environmental Adaptability of Influenza A(H9N2) Virus in the Process of Genetic Evolution. [PDF]
Jin Y +10 more
europepmc +1 more source
Integrated Assessment of Global Environmental Change with IMAGE 3.0: Model description and policy applications [PDF]
Kram, Tom +4 more
core
Embedding planetary health and sustainability in dental education: a community-engaged training model from Kenya. [PDF]
Nyachio JJ, Akama GM, Lukandu O.
europepmc +1 more source
Correction: Cardiometabolic risk factors are affected by interaction between FADS1 rs174556 variant and dietary vegetable oils in patients with diabetes: a randomized controlled trial. [PDF]
Fallah Z +10 more
europepmc +1 more source

