Results 191 to 200 of about 691,475 (266)

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Directed Evolution of Xylanase from <i>Dickeya dadantii</i> DCE-01 with Improved Enzymatic Activity. [PDF]

Polymers (Basel)
Wang R, Shi K, Zheng K, Yang Q, Xi G, Duan S, Cheng L.   +6 more
europepmc   +1 more source

Small RNA pathways in mammalian oocytes

FEBS Open Bio, EarlyView.
Three distinct small RNA pathways operate in mammalian oocytes: RNAi interference (RNAi), the microRNA (miRNA) pathway, and the PIWI‐associated RNA (piRNA) pathway. These pathways use small RNAs to guide sequence‐specific repression and contribute to oocyte biology by targeting genes and mobile elements or appear insignificant since different ...
Petr Svoboda, Josef Pasulka
wiley   +1 more source

Proximate body composition, elemental concentration and enzymatic activity analysis of Catla catla exposed to the nanoparticle (CuO-NPs) and Vitamin C. [PDF]

PLoS One
Rehman NU, Shah SQA, Naeem M, Naz H, Rafiq M.   +4 more
europepmc   +1 more source

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

FEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard, Emma‐Jayne Proctor, Jiawa Wang, Nikolas P. Johnston, Andrew Hayes, Georgia McCorkell, Haibo Yu, Jai Tree, Mark R. Davies, Mark J. Walker, Ronald Sluyter, Stephan Brouwer, Martina L. Sanderson‐Smith   +12 more
wiley   +1 more source

AFM-Based Monitoring of Enzymatic Activity of Individual Molecules of Cytochrome CYP102A1. [PDF]

Biosensors (Basel)
Ivanov YD, Bukharina NS, Shumov ID, Afonin ON, Tatur VY, Grudo AV, Archakov AI.   +6 more
europepmc   +1 more source

Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins

FEBS Open Bio, EarlyView.
Endogenously expressed fluorescent proteins can be degraded by autophagy and transported to cell nuclei via the nuclear pore complex. But in some cell lines, for example, HeLa cells which are positive for immunoreactivity of a receptor ligand, such as UCN I, in cell nuclei, fusion of autolysosome with the nuclear envelope is involved in the nuclear ...
Keiichi Ikeda
wiley   +1 more source

Intrinsically disordered region of Clr4/Suv39 regulates its enzymatic activity and ensures heterochromatin spreading. [PDF]

Nucleic Acids Res
Nakamura R, Hayashi A, Nakagawa R, Yoshimura Y, Horikoshi N, Kurumizaka H, Nakayama JI.   +6 more
europepmc   +1 more source

How phagocytic cells kill bacteria: Lessons from a professional killer

FEBS Open Bio, EarlyView.
How phagocytic cells ingest and kill bacteria has been studied for more than a century, but many questions remain unanswered. The study of the amoeba Dictyostelium discoideum brings new answers, and new questions. Professional phagocytic cells such as neutrophils and macrophages, as well as free‐living soil amoebae like Dictyostelium discoideum, employ
Otmane Lamrabet, Pierre Cosson
wiley   +1 more source

Studies on the pure fermentation and aroma contributions of <i>Torulaspora delbrueckii</i> killer yeasts with high enzymatic activity in different white wine varieties. [PDF]

Food Chem X
Jiang Z, Liu Q, Zhao X, Marie-Colette AK, Li M, Wang J.   +5 more
europepmc   +1 more source