In this work, a detection method is presented for the activity of plasma DNA methyltransferase 1 (DNMT1), namely DNMT1 Identification by Variable Activity (DIVA). DIVA can detect plasma DNMT1 at levels as low as 10−7 U mL−1, also it successfully evaluated tumor burden and predicted the neoadjuvant therapy responses of breast cancer patients.
Yingran Wang+10 more
wiley +1 more source
A σC-protein-based indirect enzyme-linked immunosorbent assay for clinical detection of antiavian reovirus antibodies. [PDF]
Yang X+10 more
europepmc +1 more source
Calpain‐2‐Mediated Endothelial Focal Adhesion Disruption in Thoracic Aortic Dissection
The elevated expression of Capn2 in endothelial cells activates focal adhesion signaling by cleaving Talin1 and assembling Itgav, which compromises the integrity of the endothelial barrier and induces vascular inflammation. Inhibition of calpain through both pharmacological and transgenic approaches has effectively suppressed the onset and progression ...
Xiaomei Teng+10 more
wiley +1 more source
Indirect Enzyme-Linked Immunosorbent Assay Based on Immunoglobulin Y Antibodies for the Evaluation of Humoral Immunity Against <i>Flavobacterium oreochromis</i> in <i>Colossoma macropomum</i>: A Preliminary Study. [PDF]
Corado MVL+9 more
europepmc +1 more source
MAGEA6 Engages a YY1‐Dependent Transcription to Dictate Perineural Invasion in Colorectal Cancer
This study investigates the role of MAGEA6 in perineural invasion (PNI) in colorectal cancer (CRC). MAGEA6 promotes CRC invasiveness by inhibiting YY1 ubiquitination, enhancing CXCL1 secretion, and recruiting Schwann cells. These findings highlight the potential of targeting the MAGEA6/YY1/CXCL1 axis for therapeutic strategies against PNI and tumor ...
Hao Wang+9 more
wiley +1 more source
Development of an enzyme-linked immunosorbent assay (ELISA) for determining neutrophil elastase (NE) - a potential useful marker of multi-organ damage observed in COVID-19 and post-Covid-19 (PCS). [PDF]
Adamiec-Mroczek J+17 more
europepmc +1 more source
Novel Bivalent mRNA‐LNP Vaccine for Highly Effective Protection against Pneumonic Plague
The study investigates mRNA‐lipid nanoparticle vaccines targeting Yersinia pestis, the causative agent of plague. Combined F1+LcrV mRNA vaccination elicited robust immune responses and protected mice against virulent wild‐type and atypical strains.
Uri Elia+12 more
wiley +1 more source
Correction to: Immunity to Non-Dengue Flaviviruses Impacts Dengue Virus Immunoglobulin G Enzyme-Linked Immunosorbent Assay Specificity in Cambodia. [PDF]
europepmc +1 more source
Digitalization of Enzyme-Linked Immunosorbent Assay with Graphene Field-Effect Transistors (G-ELISA) for Portable Ferritin Determination. [PDF]
Candia ML+3 more
europepmc +1 more source