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Enzyme-Linked Immunosorbent Assay, Elisa
The Journal of Immunology, 1972 Abstract A sensitive and simple method for the quantitative determination of antibodies is reported. Tubes coated with antigen are incubated with antiserum followed by an enzyme-labeled preparation of anti-immunoglobulin. The enzyme remaining in the tubes after washing provides a measure of the amount of specific antibodies in the serum.
E. ENGVALL, P. PERLMANN
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Enzyme-Linked Immunosorbent Assay (ELISA)
Methods in molecular biology, 2022Enzyme-linked immunosorbent assay (ELISA) is one of the most specific and straightforward assays for detecting biomolecules in research and clinics. With advances in analytical methods, ELISA assay has been constantly optimized to improve its sensitivity, and different types of ELISA are now available to detect various biomarkers. This chapter provides
Mahdis Sadat, Tabatabaei, Marya, Ahmed
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Enzyme-Linked Immunosorbent Assay (ELISA)
Methods in molecular biology, 2017 Food allergy is a public health concern especially after recognizing its constantly increased prevalence and severity. Despite careful reading of food ingredient statements, food allergic individuals may experience reactions caused by "hidden", "masked", or "contaminated" proteins that are known major allergens.
G. Konstantinou
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ACS Nano, 2020
Many proteins are present at low concentrations in biological samples and therefore, techniques for ultra-sensitive protein detection are necessary.
L. Cohen +6 more
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Many proteins are present at low concentrations in biological samples and therefore, techniques for ultra-sensitive protein detection are necessary.
L. Cohen +6 more
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Analytical Chemistry, 2020
In this work, a green enzyme-linked immunosorbent assay (ELISA) based on the single-stranded binding protein (SSB)-assisted aptamer was designed for biosensing applications.
K. Xing +5 more
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In this work, a green enzyme-linked immunosorbent assay (ELISA) based on the single-stranded binding protein (SSB)-assisted aptamer was designed for biosensing applications.
K. Xing +5 more
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, 2020
Enzyme-linked immunosorbent assay (ELISA) is a technique designed for the detection and quantification of (bio)molecules in a liquid sample.
Yuan Gao, Yingzhu Zhou, R. Chandrawati
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Enzyme-linked immunosorbent assay (ELISA) is a technique designed for the detection and quantification of (bio)molecules in a liquid sample.
Yuan Gao, Yingzhu Zhou, R. Chandrawati
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enzyme‐linked immunosorbent assay
Catalysis from A to Z, 2020-ELISA (enzyme-linked immunosorbent assay) is an important and widely used diagnostic tool in quality assurance processes in medicine, animal health, botanicals, and food and beverage manufacturing. This review describes the overview, history, protocols,
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Clickable Enzyme-Linked Immunosorbent Assay
Biomacromolecules, 2011Click chemistry is explored as a potential cost-effective and selective immobilization method for the production of an enzyme-linked immunosorbent assay (ELISA). Coatings were formulated containing either a terminal alkyne or a bicyclo[6.1.0]non-4-yne (BCN) chemical handle, and a diagnostic peptide was subsequently immobilized onto these coatings by ...
Canalle, L.A. +7 more
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Enzyme-linked immunosorbent assay (ELISA): the basics
British Journal of Hospital Medicine, 2016Shah, K., Maghsoudlou, P.
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Enzyme-Linked Immunosorbent Assay [PDF]
, 1998 Since 1971, enzyme-amplified immunoassays have been developed to enhance the detectability of antigen-antibody reactions. The most commonly applied immunoassay is the enzyme-linked immunosorbent assay (ELISA), in which the antigen-antibody complexes are adsorbed to wells in plastic microtitre plates.
Jeanne Dijkstra, Cees P. de Jager
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