Results 371 to 380 of about 671,790 (405)

Direct Competitive Enzyme-Linked Immunosorbent Assay (ELISA).

Cold Spring Harbor Protocols, 2017
The competitive enzyme-linked immunosorbent assay (ELISA) (cELISA; also called an inhibition ELISA) is designed so that purified antigen competes with antigen in the test sample for binding to an antibody that has been immobilized in microtiter plate ...
T. Kohl, C. Ascoli
semanticscholar   +1 more source

ELISA: Enzyme-Linked Immunosorbent Assay

Hospital Practice, 1978
Similar in design to radioimmunoassay, comparable in sensitivity and specificity but easier, safer, and less expensive, this new diagnostic technique uses enzyme-labeled rather than isotope-labeled reagents. The end point is a color change that can be assessed by colorimetry or with the naked eye.
openaire   +3 more sources

An enzyme-linked immunosorbent assay to characterize dengue infections where dengue and Japanese encephalitis co-circulate.

American Journal of Tropical Medicine and Hygiene, 1989
The diagnostic sensitivity and specificity of detection of anti-dengue IgM by antibody capture enzyme-linked immunosorbent assay (ELISA) was investigated in dengue infections in a variety of clinical settings. Sera from uninfected controls were uniformly
B. Innis, A. Nisalak, S. Nimmannitya, S. Kusalerdchariya, V. Chongswasdi, S. Suntayakorn, P. Puttisri, C. Hoke   +7 more
semanticscholar   +1 more source

Enzyme linked immunosorbent assay (ELISA) for paraquat

International Journal of Immunopharmacology, 1983
An Enzyme Linked Immunosorbent Assay (ELISA) has been developed for the estimation of paraquat. The amount of paraquat present in samples of human plasma was estimated in terms of the degree to which it combined with a rabbit antibody raised to a conjugate to paraquat with bovine serum albumin.
S.T. Walsh, G.E. Davies, Z. Niewola
openaire   +3 more sources

An enzyme‐linked immunosorbent assay for phytochrome

Physiologia Plantarum, 1984
A double‐antibody sandwich, enzyme‐linked immunosorbent assay has been developed for phytochrome in Avena sativa L. cv. Saladin. An immunoglobulin fraction of rabbit antiserum raised to 118 kdalton phytochrome was used with alkaline phosphatase as the enzyme label.
Brian Thomas, Susan E. Penn, Norman E. Crook   +2 more
openaire   +2 more sources

Aggregated silver nanoparticles based surface-enhanced Raman scattering enzyme-linked immunosorbent assay for ultrasensitive detection of protein biomarkers and small molecules.

Analytical Chemistry, 2015
Lowering the detection limit is critical to the design of bioassays required for medical diagnostics, environmental monitoring, and food safety regulations.
Jiajie Liang, Hongwu Liu, Caihong Huang, C. Yao, Qiangqiang Fu, Xiuqing Li, Donglin Cao, Zhi Luo, Yong Tang   +8 more
semanticscholar   +1 more source

Development of an enzyme-linked immunosorbent assay for toosendanin

Analytica Chimica Acta, 2008
Enzyme-linked immunosorbent assays (ELISAs) were developed by using polyclonal antibody for toosendanin (TSN), a biopesticide from Melai toosendan Sieb. et Zucc. Their application in the determination of this analyte in spiked cabbage, tomato and apple samples was studied.
Jing Zhang, Gang Feng, Xing Zhang, Xian Jin Liu, Xiang Yang Yu, Li Luo, Jun Tao Feng, Zhi Qing Ma   +7 more
openaire   +3 more sources

Enzyme-Linked Immunosorbent Assay: Types and Applications.

Methods in molecular biology, 2023
H. Hayrapetyan, Thao Tran, E. Tellez-Corrales, Charitha Madiraju   +3 more
semanticscholar   +1 more source

Predicting detection limits of enzyme-linked immunosorbent assay (ELISA) and bioanalytical techniques in general.

In Analysis, 2014
The detection limit is one of the most important performance parameters for bioanalytical techniques. Here we present a generic method to estimate the detection limit of biomolecular assays based on a step-by-step analysis of the assay procedure.
Shiyun Zhang, Alexa Garcia-D'Angeli, J. Brennan, Q. Huo   +3 more
semanticscholar   +1 more source

Thermometric enzyme linked immunosorbent assay: TELISA

Biochimica et Biophysica Acta (BBA) - Enzymology, 1977
A new method, thermometric enzyme linked immunosorbent assay (TELISA), for the assay of endogenous and exogenous compounds in biological fluids is described. It is based on the previously described enzyme linked immunosorbent assay technique, ELISA, but utilizes enzymic heat formation which is measured in an enzyme thermistor unit.
Bo Sanfridson, Klaus Mosbach, Carl A.K. Borrebaeck, Bo Mattiasson   +3 more
openaire   +3 more sources