Results 141 to 150 of about 3,764,590 (249)

PEAR1 Promotes Glucose Metabolism Reprogramming in Sepsis‐Associated Acute Lung Injury via AARS1‐Mediated HIF‐1α Lactylation

Advanced Science, EarlyView.
This study revealed that a PEAR1/HIF‐1α/ glycolysis/lactate/H3K18la positive feedback loop in PMVECs that drives the development of S‐ALI. Mechanistically, PEAR1 mediates the binding of HIF‐1α to AARS1, leading to the lactylation of HIF‐1α, the primary lactylation site of which is K172.
Shuai Li, Zongqing Lu, Pu Fang, Qingqing Pang, Xianghui Wu, Bo Wang, Min Liang, Jingjing Pan, Haobo Kong, Haijian Cai, Juanjuan Li, Jiaying Kang, Dongsheng Wang, Dunling Xia, Mengmeng Xu, Qinghai You   +15 more
wiley   +1 more source

A Highly Sensitive Indirect Enzyme-Linked Immunosorbent Assay (ELISA) Based on a Monoclonal Antibody Specific to Thermal Stable-Soluble Protein in Pork Fat for the Rapid Detection of Pork Fat Adulterated in Heat-Processed Beef Meatballs. [PDF]

Food Sci Anim Resour, 2023
Kim SA, Lee JE, Kim DH, Lee SM, Yang HK, Shim WB.   +5 more
europepmc   +1 more source

Hollow Cu2O Nanozymes Enhance Probiotic Therapy for Colitis via Redox Homeostasis and TXNIP/NLRP3 Inflammasome Inhibition

Advanced Science, EarlyView.
Hollow cuprous oxide (H‐Cu2O) nanozymes feature enlarged catalytic surfaces for superior reactive oxygen species (ROS) scavenging. By efficiently neutralizing mucosal ROS, H‐Cu2O directly suppresses the TXNIP/NLRP3 inflammasome axis and restores intestinal epithelial barrier integrity.
Guangzhao Wang, Jialing Cao, Pengfei Li, Yujie Wang, Shiqi Lu, Kangliang Sheng, Shan Gao, Yongzhong Wang   +7 more
wiley   +1 more source

Non-linear transformation of enzyme-linked immunosorbent assay (ELISA) measurements allows usage of linear models for data analysis. [PDF]

Virol J, 2022
Lange TM, Rotärmel M, Müller D, Mahone GS, Kopisch-Obuch F, Keunecke H, Schmitt AO.   +6 more
europepmc   +1 more source

LRG1 Drives Pathological Angiogenesis by Disrupting Neutrophil Mitochondrial Homeostasis in Bladder Cancer

Advanced Science, EarlyView.
In bladder cancer, LRG1 binds to ANXA2 to trigger mitochondrial ROS‐dependent NETosis. This pathogenic cascade actively uncouples endothelial‐mural cell interactions, driving profound vascular destabilization. Consequently, targeting the LRG1‐ANXA2 axis attenuates the neutrophil burden and induces structural vascular normalization, offering a powerful ...
Dongshan Chen, Cong Zhang, Song Xue, Yuan Zeng, Haochen Cui, Chenfei Wang, Jiayi Feng, Lei Yan, Yuanwei Zang   +8 more
wiley   +1 more source

Dot-enzyme-linked immunosorbent assay (dot-ELISA) for evaluating IgG antibody avidity in toxoplasmosis

Revista do Instituto de Medicina Tropical de São Paulo, 1997
Fabio G. Melo, Claudio L. Rossi
doaj   +1 more source

Comparison of a Commercial Enzyme-Linked Immunosorbent Assay (ELISA) with the Modified Agglutination Test (MAT) for the Detection of Antibodies against Toxoplasma gondii in a Cohort of Hunting Dogs. [PDF]

Animals (Basel), 2022
Bellatreche AY, Bouzid R, Blaizot A, Aubert D, Blaga R, Ait-Oudhia K, Le Roux D.   +6 more
europepmc   +1 more source

Self‐Assembly of Antigenic Peptide Nanofibrils Templates the Growth of Silica Nanoparticles for Nanovaccines

Advanced Science, EarlyView.
Antigenic peptides Aβ42 and E7 self‐assemble into nanofibrils; APTES and TEOS induce SiO2 NP formation on these fibrils to form SiO2@fibril nanovaccines, which activate BMDCs in vitro. In vivo, SiO2@Aβ42 fibril nanovaccines alleviate AD symptoms and clear Aβ42 plaques in APP/PS1 mice, and SiO2@E7 fibril nanovaccines inhibit tumor growth and promote ...
Xuecheng Yang, Min Li, Zhiying Yao, Yu Hu, Zhili Wang, Wendi Shi, Bingbing Sun   +6 more
wiley   +1 more source

Development and evaluation of recombinant E2 protein based IgM capture enzyme-linked immunosorbent assay (ELISA) and double antigen sandwich ELISA for detection of antibodies to Chikungunya virus. [PDF]

PLoS Negl Trop Dis, 2022
Guo M, Du S, Lai L, Wu W, Huang X, Li A, Li H, Li C, Wang Q, Sun L, Liu T, Tian T, Wang S, Liang M, Li D, Xie C, Li J.   +16 more
europepmc   +1 more source

A Glucose Metabolism‐Modulatory Nanobiohybrid Vaccine Platform Promotes Anti‐Tumor Immunity by Orchestrating Autophagy‐Dependent Cross‐Presentation and H2S‐Enhanced NLRP3 Inflammasome Signaling

Advanced Science, EarlyView.
A nanobiohybrid vaccine was designed by constructing a hydrogen‐bonded organic framework coencapsulated with glucose oxidase (GOx) and tumor antigens. This nanobiohybrid vaccine executed a GOx‐catalyzed enzyme reaction that disrupted glucose metabolism and induced redox imbalance in dendritic cells, leading to autophagy‐dependent antigen cross ...
Weidong Wang, Yimin Gong, Jianing Li, Tianze Wu, Mingli Deng, Yannan Yang   +5 more
wiley   +1 more source