Results 11 to 20 of about 272,083 (260)

Heterogeneous Immunoassay Using Channels and Droplets in a Digital Microfluidic Platform

open access: yesMicromachines, 2019
This work presents a heterogeneous immunoassay using the integrated functionalities of a channel and droplets in a digital microfluidic (DMF) platform. Droplet functionality in DMF allows for the programmable manipulation of discrete sample and reagent ...
Yuguang Liu, Ian Papautsky
doaj   +1 more source

The ELISA, Enzyme-Linked Immunosorbent Assay [PDF]

open access: yesClinical Chemistry, 2010
Featured Article: Engvall E, Perlmann P. Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry 1971;8:871–4.1 This paper was my first as a graduate student of Professor Peter Perlmann at Stockholm University.
openaire   +2 more sources

Comparison of chemiluminescence enzyme immunoassay based on magnetic microparticles with traditional colorimetric ELISA for the detection of serum α-fetoprotein

open access: yesJournal of Pharmaceutical Analysis, 2012
A chemiluminescence enzyme immunoassay based on magnetic microparticles (MmPs-CLEIA) was developed to evaluate serum α-fetoprotein (AFP) in parallel with traditional colorimetric enzyme-linked immunosorbent assay (ELISA). A systematic comparison between
Qian-Yun Zhang   +3 more
doaj   +1 more source

Three highly sensitive monoclonal antibody-based serological assays for the detection of tomato mottle mosaic virus

open access: yesPhytopathology Research, 2021
In recent years, tomato mottle mosaic virus (ToMMV) has become one of the most important viral pathogens affecting solanaceous crop production in Yunnan, Hainan, and Shandong provinces of China, often causing huge yield reductions. To provide farmers and
Xue Li   +6 more
doaj   +1 more source

Sandwich Enzyme-linked Immunosorbent Assay (ELISA) Analysis of Plant Cell Wall Glycan Connections

open access: yesBio-Protocol, 2014
Sandwich ELISA is a highly sensitive method that can be used to determine if two epitopes are part of the same macromolecule or supramolecular complex.
Valérie Cornuault, J. Knox
doaj   +1 more source

Diagnostic performance of Strongyloides-specific IgG4 detection in urine for diagnosis of human strongyloidiasis

open access: yesParasites & Vectors, 2023
Background Detection of parasite-specific IgG in urine is a sensitive method for diagnosis of strongyloidiasis and gives similar accuracy to serum IgG. However, there are no data concerning detection of IgG subclass in urine.
Phattharaphon Wongphutorn   +11 more
doaj   +1 more source

Enzyme Linked Immunosorbent Assay (ELISA)

open access: yesNatural Sciences Engineering and Technology Journal, 2021
Enzyme immunoassay (EIA) and enzyme-linked immunosorbent assay (ELISA) are both widely used as diagnostic tools in medicine and as quality control measures in many industries. ELISA has been the system of choice when testing soluble antigens and antibodies.
openaire   +2 more sources

The use of dacron plates for DOT enzyme-linked immunosorbent assay (DOT-ELISA)

open access: yesMemorias do Instituto Oswaldo Cruz, 1991
Dacron (polyethylenetherephthalate) is proposed as a matrix for dot-ELISA procedures, as an alternative to nitrocellulose. Plates of dacron were partially hydrazinolyzed and hydrazide groups introduced were converted to azide groups.
Silvia M. L. Montenegro   +3 more
doaj   +1 more source

A Cross-Sectional Serosurvey of Anti-Orthopoxvirus Antibodies in Central and Western Africa

open access: yesViruses, 2017
Since the eradication of smallpox and the subsequent discontinuation of the worldwide smallpox vaccination program, other Orthopoxviruses beside Variola virus have been increasingly representing a risk to human health.
Siv Aina J. Leendertz   +14 more
doaj   +1 more source

Comparison of enzyme linked immunosorbent assay (ELISA) with indirect immunofluorescence for detection of anti-nuclear antibody [PDF]

open access: yesJournal of Clinical and Scientific Research, 2014
Background: Detection of antinuclear antibody (ANA) is used as one of the diagnostic criteria for autoimmune rheumatic diseases (ARD). Both indirect immunofluorescence (IIF) and enzyme linked immunosorbant assay (ELISA) methods are used for this purpose.
G.L.S. Sumanth Kumar   +5 more
doaj   +1 more source

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