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Enzyme-linked immunosorbent assays: ELISA
Clinical Microbiology Newsletter, 1979Summary An effort should be made to provide standard criteria for the development of ELISA procedures and the interpretation of their results. Strict quality control will be necessary because reagents, antisera, antigens, and/or enzyme-labeled conjugates are available from many sources, and the quality of these reagents varies from excellent to very ...
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Gm typing by enzyme-linked immunosorbent assay (ELISA)
Zeitschrift f�r Rechtsmedizin, 1984A solid-phase ELISA for Gm typing is described. A mixture of anti-Gm serum (or monoclonal anti-Gm antibody) and test serum was incubated in microtiter wells coated with IgG or its fragments of appropriate Gm type. After washing of the wells, the bound antibody was detected with peroxidase-labeled second antibody.
T, Kishda, Y, Tamaki
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Enzyme-Linked Immunosorbent Assay (ELISA) for Metallothionein
1987A heterogeneous, double-antibody, fluorometric enzyme-linked immunosorbent assay (ELISA) is described for the detection and quantitation of metallothionein(MT) The protocol uses the same immunological reagents as the radioimmunoassay(RIA) developed previously in this laboratory; fluorescence replaces radioactivity for detection of the reference antigen
J S, Garvey, D G, Thomas, H J, Linton
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Quantitative enzyme-linked immunosorbent assay (ELISA) for hirudin
Journal of Immunological Methods, 1986A competitive ELISA assay has been developed that permits reproducible quantitation of the anticoagulant hirudin in buffer and urine. Coupling of peroxidase and hirudin was performed with the heterobifunctional reagent N-succinimidyl-3-(2-pyridyldithio)-propionate.
S, Spinner, G, Stöffler, E, Fink
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Enzyme-linked immunosorbent assays (ELISAs)
Critical Care Medicine, 2005Yichen, Lai +2 more
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Exercise 15: Enzyme-Linked Immunosorbent Assay (ELISA)
2021The enzyme-linked immunosorbent assay (ELISA) is a highly sensitive, rapid, relatively cheap solid-phase immunoassay that is used to screen for the presence of, and specificity of a given antibody to an antigen, or to quantitatively determine the concentrations of antibodies and soluble antigens from sources such as patient sera, immunized hosts or ...
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Enzyme- Linked Immunosorbent Assay (ELISA)
1998Stratis Avrameas, Thérèse Ternynck
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The Enzyme Linked Immunosorbent Assay (ELISA)
1987Enzyme linked immunosorbent assays (ELISA) provide sensitive methods for detecting either antigens or antibodies in biological fluids, and as such have found numerous applications, particularly in the field of clinical analysis but also in applications to research in molecular biology (see below, p. 94).
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The CRISPR–Cas toolbox for analytical and diagnostic assay development
Chemical Society Reviews, 2021Wei Feng, Qianfan Yang, Feng Li
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PD-L1 as a biomarker of response to immune-checkpoint inhibitors
Nature Reviews Clinical Oncology, 2021Mary Beth Beasley +2 more
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