Results 211 to 220 of about 293,211 (259)
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The application of enzyme-linked immunosorbent assays (ELISA) to neuropeptides
Journal of Neuroscience Methods, 1985Procedures are presented for routine evaluation of antibody specificity, titre, and quantitation of antigen levels in tissue extracts without the use of radiolabeled probes. A colorimetric, enzyme-linked immunosorbent assay (ELISA) is described for general use with neuropeptides, using neurotensin as a primary example.
L G, Davis +5 more
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An enzyme-linked immunosorbent assay (ELISA) for plasma testosterone
Journal of Steroid Biochemistry, 1985A rapid, single extraction ELISA for testosterone in plasma is described, using a standard 96 well microtitre plate. Testosterone is covalently bonded to bovine thyroglobulin and passively adsorbed in guanidine hydrochloride to the ELISA plate, giving an immobilised antigen approach which simplifies subsequent assay standardisation for steroid hormone ...
P A, Elder, J G, Lewis
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An enzyme-linked immunosorbent assay (ELISA) for plasma cortisol
Journal of Steroid Biochemistry, 1985A direct ELISA for plasma cortisol is described which is carried out in a standard 96 well microtitre plate. In this ELISA cortisol-thyroglobulin conjugate is immobilised to the microtitre plate and competes with cortisol in the standard or plasma sample for antibody binding sites.
J G, Lewis, P A, Elder
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The Enzyme Linked Immunosorbent Assay (ELISA)
1987Enzyme linked immunosorbent assays (ELISA) provide sensitive methods for detecting either antigens or antibodies in biological fluids, and as such have found numerous applications, particularly in the field of clinical analysis but also in applications to research in molecular biology (see below, p. 94).
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Enzyme-linked immunosorbent assays: ELISA
Clinical Microbiology Newsletter, 1979Summary An effort should be made to provide standard criteria for the development of ELISA procedures and the interpretation of their results. Strict quality control will be necessary because reagents, antisera, antigens, and/or enzyme-labeled conjugates are available from many sources, and the quality of these reagents varies from excellent to very ...
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The Enzyme-Linked Immunosorbent Assay (Elisa) for the Immunodiagnosis of Schistosomiasis
The American Journal of Tropical Medicine and Hygiene, 1979The enzyme-linked immunosorbent assay (ELISA) done with soluble egg antigens (SEA) of Schistosoma mansoni was utilized for the detection of infections with schistosomes. The method readily detected experimental infections in NIH outbred, New Zealand black, and New Zealand white mice by 6-10 weeks post-exposure to S. mansoni cercariae.
G V, Hillyer, I, Gómez de Rios
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An enzyme-linked immunosorbent assay (ELISA) for influenza haemagglutinin
Journal of Biological Standardization, 1982A microplate enzyme-linked immunosorbent assay is described. The assay is rapid, highly specific and reproducible. It allows the quantification of haemagglutinin in the range 0·05–0·20 μg/ml.
M I, Brady, H C, Griffiths
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Enzyme-linked immunosorbent assays (ELISAs)
Critical Care Medicine, 2005Yichen, Lai +2 more
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Enzyme- Linked Immunosorbent Assay (ELISA)
1998Stratis Avrameas, Thérèse Ternynck
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Optimality Assessment in the Enzyme-Linked Immunosorbent Assay (ELISA)
Biometrics, 1990An optimality criterion is proposed for evaluating the precision of alternative designs in the enzyme-linked immunosorbent assay. Assay profiles are represented as four-parameter logistic functions with parameter estimation based on either a weighted nonlinear regression or a simple nonlinear regression after a logarithmic transformation.
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