Results 61 to 70 of about 293,211 (259)

Quantitative determination of erlotinib in human serum using competitive enzyme-linked immunosorbent assay

open access: yesJournal of Pharmaceutical Analysis, 2018
A selective and sensitive competitive enzyme-linked immunosorbent assay (ELISA) method was developed and validated for the quantification of erlotinib in 50 µL of samples of human serum.
Yuta Yamamoto   +3 more
doaj   +1 more source

An Enzyme-Linked Immunosorbent Assay (ELISA) for Antibodies to Thyroglobulin

open access: yesExperimental Biology and Medicine, 1980
SummaryAn enzyme-linked immunosorbent assay (ELISA) was developed for the detection of thyroglobulin antibodies. The ELISA was compared with an indirect hemagglutination technique using thyroglobulin coupled to human group O erythrocytes with chromic chloride. The overall correlation between the two techniques was 0.82 (P < 0.001).
A, Voller, D E, Bidwell, C L, Burek
openaire   +2 more sources

An Adipocyte MKK3–p38β Axis Preserves Insulin Responsiveness in Mice and Is Reciprocally Altered in Human Obesity

open access: yesAdvanced Science, EarlyView.
The adipocyte MKK3–p38β axis emerges as an adaptive stress‐response pathway during obesity. Human visceral adipose tissue analyses, adipocyte‐specific mouse models, and primary adipocytes show that the MKK3–p38β axis supports insulin responsiveness by restraining p70S6K‐dependent inhibitory IRS1 feedback and preserving AKT–GLUT4 signaling. This pathway
Edgar Bernardo   +20 more
wiley   +1 more source

Microglial Deubiquitinase OTUD7B Stabilizes STAT3 to Promote Neuroinflammation and Cognitive Decline in Alzheimer's Disease

open access: yesAdvanced Science, EarlyView.
. ABSTRACT Neuroinflammation driven by microglial activation is a defining feature of Alzheimer's disease (AD), yet the molecular mechanisms sustaining this proinflammatory state remain unclear. Here, we identify the deubiquitinase OTUD7B as a critical regulator of microglial activation and AD pathology.
Luyao Li   +15 more
wiley   +1 more source

Measurement of the Memory B Cell Response via Antibodies from Activated Cells

open access: yesAntibodies
Background/Objectives: The body’s immune response to infections and vaccination leads to the formation of memory B cells (MBCs), which protect against future infections.
Caroline Rockstroh   +3 more
doaj   +1 more source

Comparison of a rapid immunochromatography assay with an enzyme linked immunosorbent assay (ELISA) for anti-dengue virus IgM detection

open access: yesSri Lankan Journal of Infectious Diseases, 2014
ObjectivesThe current study was undertaken to compare the sensitivity, specificity, positive predictive value and negative predictive values of a dengue IgM rapid immunochromatography (ICT) assay with a dengue IgM capture ELISA for the detection of anti ...
T Senaratne   +3 more
doaj   +1 more source

Biofilm‐Binding Phages Enhance Biofilm Eradication by Synergistic Photothermal and Photodynamic Therapy

open access: yesAdvanced Science, EarlyView.
Engineered phage‐based trifunctional nanofibers are developed for synergistic biofilm eradication. Biofilm‐binding phages, armed with AuNPs and TCPP, serve as both warheads and scaffolds, endowing the phototherapy agents with biofilm‐anchoring ability. Upon light irradiation, AuNPs induce photothermal therapy, while TCPP generates 1O2 for photodynamic ...
Ying Cao   +9 more
wiley   +1 more source

Fluobodies against Bioactive Natural Products and their Application in Fluorescence-Linked Immunosorbent Assay

open access: yesAntibodies, 2012
An enzyme-linked immunosorbent assay (ELISA) using monoclonal antibody (MAb), Fab antibody, and single-chain variable fragment (scFv) antibody has become one of the most promising analytical methods owing to its rapidity, sensitivity, and reliability ...
Seiichi Sakamoto   +6 more
doaj   +1 more source

An Activity‐Reinforced P‐Modified Single‐Atom Cu Nanozyme Potentiates IDO Blockade‐Induced Tumor Radiotherapy

open access: yesAdvanced Science, EarlyView.
A phosphorus‐modified single‐atom copper nanozyme delivering the IDO1 inhibitor 1‐methyl‐tryptophan enhances breast cancer radioimmunotherapy by simultaneously relieving hypoxia and inducing immunogenic cell death through dual enzyme activities while blocking tryptophan metabolism to suppress regulatory T cell function.
Wei Zhao   +11 more
wiley   +1 more source

Actual condition survey regarding mismatch of measurements between radioimmunoassay and enzyme‐linked immunosorbent assay tests for anti‐glutamic acid decarboxylase antibody in real‐world clinical practice

open access: yesJournal of Diabetes Investigation, 2019
Anti‐glutamic acid decarboxylase antibody (GADA) is an important islet cell‐associated autoantibody for the diagnosis of autoimmune type 1 diabetes mellitus.
Yoichi Oikawa   +4 more
doaj   +1 more source

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