Purification and Characterization of Polyhydroxyalkanoate Synthase from Extremely Halophilic Archaeon <i>Haloferax mediterranei</i>: Key Enzyme of Biodegradable Plastic Synthesis. [PDF]
Bioengineering (Basel)Alsafadi D, Ghalawinji Y, Khalili FI.
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LIBERATION OF LYSINE BY ACID AND ENZYMES FROM HEATED LYSINE-GLUCOSE MIXTURE
, 1951 D.R. Clandinin +3 more
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HSP70 governs permeability and mechanotransduction in primary human endothelial cells
FEBS Open Bio, EarlyView.HSP70 chemical inhibition reduces endothelial cell proliferation and increases permeability, the latter supported by normal interendothelial junctional protein distribution. HSP70 also plays a role in shear stress response, a hemodynamic force naturally present in blood vessels and correlated with vessel protection.
Andrea Pinto‐Martinez +5 more
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A method for predicting enzyme substrate specificity residues using homologous sequence information. [PDF]
Protein SciMori S, Niide T, Toya Y, Shimizu H.
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Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein
FEBS Open Bio, EarlyView.This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
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Prediction of enzyme function using an interpretable optimized ensemble learning framework. [PDF]
Chem SciDhibar S, Basak S, Jana B.
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THE EFFECT OF DEHYDRATION ON THE PANCREATIC AND INTESTINAL ENZYMES
, 1934 John Ross, Margaret Shaw
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Thrombolytic proteins profiling: High‐throughput activity, selectivity, and resistance assays
FEBS Open Bio, EarlyView.We present optimized biochemical protocols for evaluating thrombolytic proteins, enabling rapid and robust screening of enzymatic activity, inhibition resistance, and fibrin affinity, stimulation, and selectivity. The outcome translates to key clinical indicators such as biological half‐life and bleeding risk. These assays streamline the development of
Martin Toul +3 more
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Dynamics of single enzymes confined inside a nanopore. [PDF]
RSC Chem BiolGalenkamp NS, van den Noort M, Maglia G.
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