Results 361 to 370 of about 6,529,404 (392)

Molecular cloning of human D-dopachrome tautomerase cDNA: N-terminal proline is essential for enzyme activation.

Biochemical and Biophysical Research Communications - BBRC, 1998
D-Dopachrome tautomerase converts 2-carboxy-2,3-dihydroindole-5,6-quinone (D-dopachrome) into 5,6-dihydroxyindole. This protein has an amino acid sequence that is highly homologous with that of macrophage migration inhibitory factor (MIF), which has the ...
J. Nishihira, Mami Fujinaga, Takeshi Kuriyama, Masaki Suzuki, Hiroshi Sugimoto, Atsushi Nakagawa, Isao Tanaka, Masaharu Sakai   +7 more
semanticscholar   +1 more source

Measuring the Enzyme Activity of Arabidopsis Deubiquitylating Enzymes

2016
Deubiquitylating enzymes, or DUBs, are important regulators of ubiquitin homeostasis and substrate stability, though the molecular mechanisms of most of the DUBs in plants are not yet understood. As different ubiquitin chain types are implicated in different biological pathways, it is important to analyze the enzyme characteristic for studying a DUB ...
Kamila Kalinowska, Erika Isono, Marie-Kristin Nagel   +2 more
openaire   +3 more sources

Measurement of Enzyme Activity

1982
Most people approaching the problem of enzyme purification will be following a standard procedure for measuring enzyme activity. But in some cases, it may be useful to develop an alternative assay, for instance, a rapid spectrophotometric method to avoid queuing for the use of a scintillation counter.
openaire   +2 more sources

Maintenance of Active Enzymes

1982
A key requirement in enzyme work is to know the pH at all times; to control the pH a buffer is essential. It is also important to know the characteristics of the buffer being used and to have on hand a range of different buffers so that one most suited to the application is available.
openaire   +2 more sources

Enzyme Activity and Firmness in Tomatoes

2018
The effect of storage at 3 ‘safe’ temperatures and one chilling temperature on the firmness, water loss and activity of selected enzyme systems (PG) of tomatoes (cv. Tradiro), harvested at two stages of maturity, was experimentally determined. Models built on assumed but plausible reaction mechanisms explain very well the behavior of these product ...
Tijskens, L.M.M., van Dijk, C.
openaire   +3 more sources

[5] Measurement of enzyme activity

1990
Publisher Summary This chapter describes the development of methods for the assay of enzyme activity in a cell lysate or in a partially purified enzyme preparation. These assays are also applicable during purification and for purified enzymes as well. The first component of an assay is the reaction mixture.
openaire   +3 more sources

Regulation of Enzyme Activity

2018
Most organisms, however evolutionarily distant, contain a set of common metabolites. But their intracellular concentrations are unique to each individual species. This metabolic identity of an organism is the consequence of quantitative differences in relevant enzyme properties and their associated regulation.
openaire   +2 more sources

Angiogenic activity of enzymes [PDF]

Nature, 1994
Tatsuhiko Furukawa, Tomoyuki Sumizawa, Kazutaka Miyadera, Yuji Yamada, Kazutaka Yamada, Misako Haraguchi, Shin-ichi Akiyama, Katsuo Uemura   +7 more
openaire   +2 more sources

Palladium-mediated enzyme activation suggests multiphase initiation of glycogenesis

Nature, 2018
Matthew K. Bilyard, H. Bailey, L. Raich, Maria A. Gafitescu, Takuya Machida, Javier Iglesias-Fernández, S. S. Lee, Christopher D. Spicer, C. Rovira, W. Yue, B. G. Davis   +10 more
semanticscholar   +1 more source

Chapter 7 Measurement of Enzyme Activity

2009
To study and understand the nature of living cells, scientists have continually employed traditional biochemical techniques aimed to fractionate and characterize a designated network of macromolecular components required to carry out a particular cellular function.
Malik M. Keshwani, Thomas K. Harris
openaire   +2 more sources