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Enzyme-Linked Immunosorbent Assay
Journal of Immunoassay, 2000(2000). Enzyme-Linked Immunosorbent Assay. Journal of Immunoassay: Vol. 21, No. 2-3, pp. 165-209.
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A fluorescent assay for proteolytic enzymes
Analytical Biochemistry, 1973Abstract A method is described which permits the assay of proteolytic enzyme activity on protein substrates without precipitation or filtration steps, utilizing a fluorescent reagent which is specific for primary amines. The assay is about 100 times more sensitive than the Lowry method, much faster and less complicated.
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Capillary Electrophoretic Enzyme Assays
2013In the past years, capillary electrophoresis has become a frequently used technique for enzyme assays due to the high separation efficiency and versatility as well as small sample size and low consumption of chemicals. The capillary electrophoresis assays can be divided into two general categories: pre-capillary (or offline) assays and in-capillary (or
Gerhard K E, Scriba +3 more
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Automated Enzyme Assay of Antithrombin
Annals of Clinical Biochemistry: International Journal of Laboratory Medicine, 1980The conditions for the automated assay of plasma antithrombin activity over the clinically relevant range of 25–250% using the chromogenic substrate S2238 and the Gilford 3800 enzyme rate analyser are reported.
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Optimizing coupled enzyme assays
Analytical Biochemistry, 1979Abstract Equations are given for minimizing the cost and calculating the maximum practical rates for coupled assays when two or more coupling enzymes are involved, and a lag of a predetermined size can be tolerated. The lag caused by obligatory mutarotation of an intermediate is also calculated.
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