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Metal-Organic Frameworks-Based Immobilized Enzyme Microreactors Integrated with Capillary Electrochromatography for High-Efficiency Enzyme Assay.

Analytical Chemistry, 2022
Enzyme assays are important for studying enzyme-mediated biochemical reactions and for clinical diagnosis and drug development. The technique of an immobilized enzyme microreactor (IMER) integrated with capillary electrophoresis (CE) has been frequently ...
R. Liu   +6 more
semanticscholar   +1 more source

Enzyme assays

Chem. Commun., 2008
Enzyme assays are analytical tools to visualize enzyme activities. In recent years a large variety of enzyme assays have been developed to assist the discovery and optimization of industrial enzymes, in particular for "white biotechnology" where selective enzymes are used with great success for economically viable, mild and environmentally benign ...
Jean-Louis, Reymond   +2 more
openaire   +2 more sources

Metal and Metal Oxide Nanoparticles to Enhance the Performance of Enzyme-Linked Immunosorbent Assay (ELISA)

, 2020
Enzyme-linked immunosorbent assay (ELISA) is a technique designed for the detection and quantification of (bio)molecules in a liquid sample.
Yuan Gao, Yingzhu Zhou, R. Chandrawati
semanticscholar   +1 more source

Polyphosphate Chain Length Determination in the Range of Two to Several Hundred P-Subunits with a New Enzyme Assay and 31P NMR.

Analytical Chemistry, 2019
Currently, 31P NMR is the only analytical method that quantitatively determines the average chain length of long inorganic polyphosphate (>80 P-subunits).
Jonas Johannes Christ   +2 more
semanticscholar   +1 more source

Assay of enzyme effectors

Talanta, 1987
The effects of various classes of organic compounds and of metal ions on the catalytic activity of horseradish peroxidase in hydrogen peroxide-catalysed o-dianisidine oxidation and, on the activity of alkaline phosphatase in p-nitrophenyl phosphate hydrolysis have been studied.
V.V. Kutcheryaeva   +2 more
openaire   +3 more sources

An enzyme assay for acetylcholine

Biochemical Pharmacology, 1969
Abstract A specific and sensitive enzymatic assay for acetylcholine has been developed. The acetylcholine was isolated from tissue extracts by electrophoresis, hydrolyzed to choline, and reacetylated by the transfer of the acetyl- 14 C group of acetyl- 14 C-coenzyme A to the choline molecule by the enzyme, choline acetyltransferase.
M.E. Feigenson, J.K. Saelens
openaire   +3 more sources

Easy cellulose-based strips designed based on a fast and sensitive enzyme colorimetric assay for the low concentration of glucose detection.

Analytical Chemistry, 2019
Compared with traditional filter paper, high-purity cellulose membrane prepared via green pathway was used to attempt to enhance their performance of glucose detection.
Xiaogang Luo   +4 more
semanticscholar   +1 more source

Enzyme assays

Current Opinion in Biotechnology, 1991
The past year or so has seen the development of new enzyme assays, as well as the improvement of existing ones. Assays are becoming more rapid and sensitive as a result of modifications such as amplification of the enzyme product(s). Recombinant DNA technology is now being recognized as a particularly useful tool in the search for improved assay ...
openaire   +2 more sources

An Enzyme Cascade-Triggered Fluorogenic and Chromogenic Reaction Applied in Enzyme Activity Assay and Immunoassay.

Analytical Chemistry, 2018
An enzyme cascade-triggered reaction with novel signal generation mechanism is beneficial for the development and insight of the enzyme cascade, which is extensively used for signal transduction in potential applications.
Jiahui Zhao   +5 more
semanticscholar   +1 more source

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