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Rapid electrochemical enzyme assay with enzyme-free calibration.
Analytical Chemistry, 2013The internally calibrated electrochemical continuous enzyme assay (ICECEA, patent pending) was developed for the fast determination of enzyme activity unit (U).
Mao-gen Zhang, Sushma Karra, W. Gorski
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ACS Applied Materials and Interfaces, 2016
Protein-inorganic nanoflowers, composed of protein and copper(II) phosphate (Cu3(PO4)2), have recently grabbed people's attention. Because the synthetic method requires no organic solvent and because of the distinct hierarchical nanostructure, protein ...
Tianxiang Wei +4 more
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Protein-inorganic nanoflowers, composed of protein and copper(II) phosphate (Cu3(PO4)2), have recently grabbed people's attention. Because the synthetic method requires no organic solvent and because of the distinct hierarchical nanostructure, protein ...
Tianxiang Wei +4 more
semanticscholar +1 more source
Allosteric indicator displacement enzyme assay for a cyanogenic glycoside.
Chemistry, 2013Indicator displacement assays (IDAs) represent an elegant approach in supramolecular analytical chemistry. Herein, we report a chemical biosensor for the selective detection of the cyanogenic glycoside amygdalin in aqueous solution.
D. Jose, Martin Elstner, A. Schiller
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Immunochemical assay of enzymes
TrAC Trends in Analytical Chemistry, 1986Abstract Immunochemical assays of enzymes are sometimes advantageous when compared with assaying enzymes according to their catalytic activity. They allow the determination of an enzyme in the presence of an inhibitor, in the inactivated or zymogen form, as well as the determination of its origin and ontological relationship.
J. Káš, P. Rauch, L. Fukal
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Recent advances in enzyme assays [PDF]
Trends in Biotechnology, 2004 Enzyme assays for high-throughput screening and enzyme engineering, which are often based on derivatives of coumarin, nitrophenol, fluorescein, nitrobenzofurazane or rhodamine dyes, can be divided into two categories: those that depend on labelled substrates, and those that depend on sensing the reactions of unmodified substrates.
Jean-Philippe Goddard +1 more
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Analytical Chemistry, 2015
Homogenous electrochemical biosensing strategies have attracted substantial attention, because of their advantages of being immobilization-free and having rapid response and improved recognition efficiency, compared to heterogeneous biosensors; however ...
Ting Hou, Wei Li, Xiaojuan Liu, Feng Li
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Homogenous electrochemical biosensing strategies have attracted substantial attention, because of their advantages of being immobilization-free and having rapid response and improved recognition efficiency, compared to heterogeneous biosensors; however ...
Ting Hou, Wei Li, Xiaojuan Liu, Feng Li
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Enzyme‐Linked Immunosorbent Assays
Current Protocols in Immunology, 1992AbstractThis unit describes six different ELISA systems for the detection of specific antibodies, soluble antigens, or cell‐surface antigens. In all six systems, soluble reactants are removed from solution after specifically binding to solid‐phase reactants.
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Enzyme-Coupled Assays for Proteases
Analytical Biochemistry, 1996We have developed a general strategy for assaying proteases that does not require the use of fluorogenic, chromogenic, or radiolabeled peptide substrates. The endo- or exoproteolytic hydrolysis of simple peptides can be followed spectrophotometrically by coupling the proteolytic event via enzyme-catalyzed reactions to a chromogenic redox dye.
John V. Schloss, Brian E. Cathers
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Automated Enzyme Assay of Antithrombin [PDF]
Annals of Clinical Biochemistry: International Journal of Laboratory Medicine, 1980 The conditions for the automated assay of plasma antithrombin activity over the clinically relevant range of 25–250% using the chromogenic substrate S2238 and the Gilford 3800 enzyme rate analyser are reported.
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Enzyme-Linked Immunosorbent Assay: Types and Applications.
Methods in molecular biology, 2023H. Hayrapetyan +3 more
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