Results 351 to 360 of about 5,306,282 (389)
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2003
Reporter genes code for proteins that have a unique enzymatic activity and are used to assess the transcriptional properties of DNA elements. The use of reporter genes in transgenic animals provides a rapid method for the detection of transgene expression, which is easily distinguishable from expression of the corresponding endogenous gene of the ...
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Reporter genes code for proteins that have a unique enzymatic activity and are used to assess the transcriptional properties of DNA elements. The use of reporter genes in transgenic animals provides a rapid method for the detection of transgene expression, which is easily distinguishable from expression of the corresponding endogenous gene of the ...
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Assay of the enzymes of glutathione biosynthesis
Analytical Biochemistry, 2022This article is dedicated to the late long-time Editor-in-Chief of Analytical Biochemistry, William Jakoby. As a graduate student, I remember reading many articles in Analytical Biochemistry and Methods in Enzymology, both volumes that Bill edited. I first met him as a graduate student presenting at the American Society of Biochemistry (and Molecular ...
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Co₃O₄ nanoparticles with multi-enzyme activities and their application in immunohistochemical assay.
ACS Applied Materials and Interfaces, 2014Co3O4 nanoparticles (Co3O4 NPs), synthesized by the coprecipitation method, showed intrinsic catalase-like, peroxidase-like, and SOD-like activity. The catalytic activity of Co3O4 NPs was much higher than analogous Fe3O4 NPs.
Jinlai Dong +6 more
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Analysis of Cell Viability by the Lactate Dehydrogenase Assay.
Cold Spring Harbor Protocols, 2018A common method for determining cytotoxicity is based on measuring the activity of cytoplasmic enzymes released by damaged cells. Lactate dehydrogenase (LDH) is a stable cytoplasmic enzyme that is found in all cells. LDH is rapidly released into the cell
Priti Kumar +2 more
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Enzymic assay for spermine and spermidine
Analytical Biochemistry, 1966Abstract This paper describes a sensitive enzymic micromethod for the quantitative assay of spermine and spermidine. The method is based on the oxidation of the polyamines by serum amine oxidase. The aminoaldehydes produced during the oxidation are assayed by N -methyl-2-benzothiazolone hydrazone hydrochloride, at 660 mμ.
B. Reches, Uriel Bachrach
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Enzyme-Linked Immunosorbent Assay (ELISA).
Methods in molecular biology, 2022M. Tabatabaei, Marya Ahmed
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In Analysis, 2014
The detection limit is one of the most important performance parameters for bioanalytical techniques. Here we present a generic method to estimate the detection limit of biomolecular assays based on a step-by-step analysis of the assay procedure.
Shiyun Zhang +3 more
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The detection limit is one of the most important performance parameters for bioanalytical techniques. Here we present a generic method to estimate the detection limit of biomolecular assays based on a step-by-step analysis of the assay procedure.
Shiyun Zhang +3 more
semanticscholar +1 more source
Spectrophotometric standardization of enzyme assays
Clinica Chimica Acta, 1972Abstract In the absence of reliable standard reference material for clinical enzymology at a reasonable purity and/or stability and/or cost, many enzyme units have been based on spectrophotometric absorbance units. To insure accuracy, it is thus necessary to directly standardize the instruments resulting in an indirect standardization of the enzyme ...
S.Lawrence Jacobs +2 more
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2018
Enzymology is a quantitative and exact science. Therefore it is important to understand how enzyme activity is measured and presented. A robust and reliable measure of the progress of an enzyme-catalyzed reaction is first and foremost requirement. Like with any other chemical reaction, progress of an enzyme-catalyzed reaction can be monitored either by
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Enzymology is a quantitative and exact science. Therefore it is important to understand how enzyme activity is measured and presented. A robust and reliable measure of the progress of an enzyme-catalyzed reaction is first and foremost requirement. Like with any other chemical reaction, progress of an enzyme-catalyzed reaction can be monitored either by
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A fluorescent assay for proteolytic enzymes
Analytical Biochemistry, 1973Abstract A method is described which permits the assay of proteolytic enzyme activity on protein substrates without precipitation or filtration steps, utilizing a fluorescent reagent which is specific for primary amines. The assay is about 100 times more sensitive than the Lowry method, much faster and less complicated.
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