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Nanoparticles for Use in Enzyme Assays
ChemBioChem, 2016AbstractNanoparticles (NPs) have created new ways to enhance the performance of classical biosensors in analytical sciences. NPs with unprecedented physiochemical properties can serve both as excellent carriers of bioreceptors and as signal enhancers, leading to improved assay platforms with high sensitivity and selectivity.
Young-Pil Kim
exaly +3 more sources
Chem. Commun., 2008
Enzyme assays are analytical tools to visualize enzyme activities. In recent years a large variety of enzyme assays have been developed to assist the discovery and optimization of industrial enzymes, in particular for "white biotechnology" where selective enzymes are used with great success for economically viable, mild and environmentally benign ...
Reymond, Jean-Louis +2 more
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Enzyme assays are analytical tools to visualize enzyme activities. In recent years a large variety of enzyme assays have been developed to assist the discovery and optimization of industrial enzymes, in particular for "white biotechnology" where selective enzymes are used with great success for economically viable, mild and environmentally benign ...
Reymond, Jean-Louis +2 more
openaire +4 more sources
Talanta, 1987
The effects of various classes of organic compounds and of metal ions on the catalytic activity of horseradish peroxidase in hydrogen peroxide-catalysed o-dianisidine oxidation and, on the activity of alkaline phosphatase in p-nitrophenyl phosphate hydrolysis have been studied.
I F, Dolmanova +2 more
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The effects of various classes of organic compounds and of metal ions on the catalytic activity of horseradish peroxidase in hydrogen peroxide-catalysed o-dianisidine oxidation and, on the activity of alkaline phosphatase in p-nitrophenyl phosphate hydrolysis have been studied.
I F, Dolmanova +2 more
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An enzyme assay for acetylcholine
Biochemical Pharmacology, 1969Abstract A specific and sensitive enzymatic assay for acetylcholine has been developed. The acetylcholine was isolated from tissue extracts by electrophoresis, hydrolyzed to choline, and reacetylated by the transfer of the acetyl- 14 C group of acetyl- 14 C-coenzyme A to the choline molecule by the enzyme, choline acetyltransferase.
M E, Feigenson, J K, Saelens
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1992
Abstract Enzyme assays are among the most frequently performed procedures in biochemistry and are routinely used to estimate the amount of enzyme present in a cell or tissue, to follow the purification of an enzyme, or to determine the kinetic parameters of a system. The range of techniques used to measure the rate of an enzyme-catalysed
TSUJI AKIO +2 more
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Abstract Enzyme assays are among the most frequently performed procedures in biochemistry and are routinely used to estimate the amount of enzyme present in a cell or tissue, to follow the purification of an enzyme, or to determine the kinetic parameters of a system. The range of techniques used to measure the rate of an enzyme-catalysed
TSUJI AKIO +2 more
openaire +4 more sources
Current Opinion in Biotechnology, 1991
The past year or so has seen the development of new enzyme assays, as well as the improvement of existing ones. Assays are becoming more rapid and sensitive as a result of modifications such as amplification of the enzyme product(s). Recombinant DNA technology is now being recognized as a particularly useful tool in the search for improved assay ...
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The past year or so has seen the development of new enzyme assays, as well as the improvement of existing ones. Assays are becoming more rapid and sensitive as a result of modifications such as amplification of the enzyme product(s). Recombinant DNA technology is now being recognized as a particularly useful tool in the search for improved assay ...
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Enzyme‐Linked Immunosorbent Assays
Current Protocols in Immunology, 1992AbstractThis unit describes six different ELISA systems for the detection of specific antibodies, soluble antigens, or cell‐surface antigens. In all six systems, soluble reactants are removed from solution after specifically binding to solid‐phase reactants.
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2003
Reporter genes code for proteins that have a unique enzymatic activity and are used to assess the transcriptional properties of DNA elements. The use of reporter genes in transgenic animals provides a rapid method for the detection of transgene expression, which is easily distinguishable from expression of the corresponding endogenous gene of the ...
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Reporter genes code for proteins that have a unique enzymatic activity and are used to assess the transcriptional properties of DNA elements. The use of reporter genes in transgenic animals provides a rapid method for the detection of transgene expression, which is easily distinguishable from expression of the corresponding endogenous gene of the ...
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Enzyme-Coupled Assays for Proteases
Analytical Biochemistry, 1996We have developed a general strategy for assaying proteases that does not require the use of fluorogenic, chromogenic, or radiolabeled peptide substrates. The endo- or exoproteolytic hydrolysis of simple peptides can be followed spectrophotometrically by coupling the proteolytic event via enzyme-catalyzed reactions to a chromogenic redox dye.
B E, Cathers, J V, Schloss
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HORMONE AND ENZYME ASSAYS IN PREGNANCY
Acta Endocrinologica, 1974ABSTRACT The cystine-aminopeptidase (CAP)1) activity of placental origin (P-CAP) and of tissue origin (T-CAP) has been investigated in plasma from non-pregnant women, during normal pregnancy, in pregnancies complicated with severe pre-eclampsia and in plasma from the umbilical cord.
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