Results 21 to 30 of about 358,322 (261)

Development of a Simple Pretreatment Immunoassay Based on an Organic Solvent-Tolerant Nanobody for the Detection of Carbofuran in Vegetable and Fruit Samples

Biomolecules, 2019
Nanobodies are one-tenth the size of conventional antibodies and are naturally obtained from the atypical heavy-chain-only antibodies present in camelids.
Jin-ru Zhang, Yu Wang, Jie-xian Dong, Jin-yi Yang, Yu-qi Zhang, Feng Wang, Rui Si, Zhen-lin Xu, Hong Wang, Zhi-li Xiao, Yu-dong Shen   +10 more
doaj   +1 more source

Using Enzyme-Linked Aptamer Assay to Detect Hepatitis B Surface Antigen in Comparison to Enzyme-Linked Immunosorbent Assay

مجله دانشکده پزشکی اصفهان, 2018
Background: Clinical diagnostic kits for detecting hepatitis B are based on antibodies, and have inefficient sensitivity, stability, and cost. Therefore, researches about aptamers and using them instead of antibodies may make these defects up.
Hamidreza Mianesaz, Hossein Khanahmad, Mina Mirian, Maryam Boshtam, Seyed Nezamoddin Hoseini   +4 more
doaj   +1 more source

Development of Immunoassay Based on Monoclonal Antibody Reacted with the Neonicotinoid Insecticides Clothianidin and Dinotefuran

Sensors, 2012
Enzyme-linked immunosorbent assay (ELISA) based on a monoclonal antibody (MoAb) was developed for the neonicotinoid insecticide clothianidin. A new clothianidin hapten (3-[5-(3-methyl-2-nitroguanidinomethyl)-1,3-thiazol-2-ylthio] propionic acid) was ...
Seiji Iwasa, Mikiko Uchigashima, Shigekazu Ito, Eiki Watanabe, Shiro Miyake   +4 more
doaj   +1 more source

Salivary chromogranin A levels relate to fatigability after waking up

MethodsX
This study aimed to assess fatigue using a noninvasive, quantitative, and simple method using salivary chromogranin A (CgA). In total, 171 adults participated in this study.
Kentaro Taniguchi, Naoya Okumura, Naoya Jinno, Akitoshi Seiyama, Akito Shimouchi   +4 more
doaj   +1 more source

Spreading of dengue virus infection cases in Malda district of West Bengal, India during COVID-19 pandemic situation (2020 to 2022): A cross-sectional study [PDF]

Journal of Research in Clinical Medicine
Introduction: One of the most widespread arboviral infections is caused by the dengue virus (Family: Flaviviridae) having a positive sense single-stranded RNA genome.
Puranjay Saha, Poulami Saha, Subhayan Das Gupta, Dwiptirtha Chattopadhyay, Subham Das   +4 more
doaj   +1 more source

Epitope Mapping of Monoclonal Antibodies to Calreticulin Reveals That Charged Amino Acids Are Essential for Antibody Binding

Antibodies, 2021
Calreticulin is a chaperone protein, which is associated with myeloproliferative diseases. In this study, we used resin-bound peptides to characterize two monoclonal antibodies (mAbs) directed to calreticulin, mAb FMC 75 and mAb 16, which both have ...
Ann Christina Bergmann, Cecilie Kyllesbech, Rimantas Slibinskas, Evaldas Ciplys, Peter Højrup, Nicole Hartwig Trier, Gunnar Houen   +6 more
doaj   +1 more source

Enzyme-Linked Immunosorbent Assay

, 2023
The current state of enzyme immunoassay of medicinal substances is considered. The main methods for obtaining and purifying specific antibodies, immunogens, enzyme conjugates are described. Examples of definitions are given medicinal substances by enzyme immunoassay, lists the possible causes of errors in the determination of medicinal substances by ...
Rooma Devi, Aman Chauhan, Simmi Kharb, Chandra Shekhar Pundir   +3 more
openaire   +3 more sources

Development of a New Monoclonal Antibody against Brevetoxins in Oyster Samples Based on the Indirect Competitive Enzyme-Linked Immunosorbent Assay

Foods, 2021
The consumption of shellfish contaminated with brevetoxins, a family of ladder-frame polyether toxins formed during blooms of the marine dinoflagellate Karenia brevis, can cause neurotoxic poisoning, leading to gastroenteritis and neurotoxic effects.
Xiya Zhang, Mingyue Ding, Chensi Zhang, Yexuan Mao, Youyi Wang, Peipei Li, Haiyang Jiang, Zhanhui Wang, Xuezhi Yu   +8 more
doaj   +1 more source

A comparative effectiveness research of azathioprine and cyclophosphamide on the clinical and serological response in pemphigus vulgaris

Indian Journal of Dermatology, 2016
Context: A prospective study was carried out to examine the efficacy of cyclophosphamide and azathioprine in pemphigus vulgaris. Aims: To compare the clinical and serological effect of azathioprine and cyclophosphamide in pemphigus patients.
Kabir Sardana, Pooja Agarwal, Shivani Bansal, Beena Uppal, Vijay K Garg   +4 more
doaj   +1 more source

Embedded optofluidic biosensing platform for enzyme-linked immunosorbent assay

Sensing and Bio-Sensing Research
With the growing demand for applications in disease diagnostics, bioanalysis, and health monitoring, developing efficient biosensing systems for fast detections and trace analysis of biomarkers is of great significance.
Jie Zhang, Yuyao Li, Yuan Fang, Junzhe Wang, Erhan Zhuo, Jiekai Zhuo, Xia Ouyang, Zidan Gong   +7 more
doaj   +1 more source