FEBS Open Bio, EarlyView.The C. elegans tetraspanin‐7 (tsp‐7) is a homologue of human CD63, which is a negative regulator of autophagy. The C. elegans strain, tm5761, has a dysfunctional (knockout) tsp‐7 gene. When compared to the wild‐type strain, the tm5761 strain shows increased: life‐ and health‐span; thermotolerance, and stress‐induced locomotion.
Brogan Jones +2 more
wiley +1 more source
A Reverse Vaccinology and Immunoinformatic Approach for the Designing of a Novel mRNA Vaccine Against Stomach Cancer Targeting the Potent Pathogenic Proteins of <i>Helicobacter pylori</i>. [PDF]
Bioinform Biol InsightsBarua A, Masum MHU, Mahdeen AA.
europepmc +1 more source
FEBS Open Bio, EarlyView.We introduce an immunohistochemistry method to measure autophagy flux, highlighting the active degradation and recycling of cellular waste. This cost‐effective approach uses tissue samples to track key markers like LC3 and SQSTM1, revealing how cells maintain health or respond to diseases such as cancer. It bridges the gap between research and clinical
Shahla Shojaei +6 more
wiley +1 more source
A dominant, pan-DR binding epitope of Der p 1 in house dust mite allergy induces tolerance in HLA-DR4 transgenic mice. [PDF]
Front ImmunolStreeter HB +4 more
europepmc +1 more source
FEBS Open Bio, EarlyView.Heterologous expression of membrane transporters in cultured cells is essential for functional characterization, but is sometimes limited by low activity. Our study compares the HDAC inhibitors butyrate, VPA and SAHA to enhance transport activity. We propose to replace butyrate by SAHA: it is equally effective, devoid of repulsive odor, costs less, and
Svenja Flögel +4 more
wiley +1 more source
Protocol for designing a peptide-based multi-epitope vaccine targeting monkeypox using reverse vaccine technology. [PDF]
STAR ProtocKumar A +12 more
europepmc +1 more source
Establishment and biological characterization of radioresistant colorectal cancer cell lines
FEBS Open Bio, EarlyView.Under ionizing radiation exposure, radiation‐sensitive cancer cells exhibit oxidative stress and DNA damage, while radiation‐resistant cancer cells exhibit strong antioxidant properties and DNA damage repair. Radiotherapy resistance is a major cause of recurrence and metastasis in colorectal cancer (CRC).
Tian‐Yin Qu +10 more
wiley +1 more source
Design and evaluation of a poly-epitope based vaccine for the induction of influenza A virus cross-reactive CD8 + T cell responses. [PDF]
Sci RepDam S +5 more
europepmc +1 more source
FEBS Open Bio, EarlyView.Measuring AMP‐activated protein kinase (AMPK) activity in vitro is crucial for testing AMPK activators or inhibitors with therapeutic potential. Here, we report an enzyme‐linked immunosorbent assay (ELISA)‐based AMPK activity assay with simple steps and high sensitivity, which offers a simple, robust, and cost‐effective alternative to traditional ...
Trezze P. Nguyen, Shangze Lyu, Yang Liu
wiley +1 more source
Development of a multi-epitope vaccine from outer membrane proteins and identification of novel drug targets against <i>Francisella tularensis</i>: an <i>In Silico</i> approach. [PDF]
Front ImmunolMoradkasani S +7 more
europepmc +1 more source