Results 81 to 90 of about 4,237,260 (302)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

open access: yesFEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong   +3 more
wiley   +1 more source

A Discrete Numerical Solution of The SIR Model with Horizontal and Vertical Transmission

open access: yesCauchy: Jurnal Matematika Murni dan Aplikasi, 2019
The aim of this paper is to is to generalize the SIR model with horizontal and vertical transmission. In this paper, we develop the discrete version of the model. We use Euler method to approximate numerical solution of the model.
Trija Fayeldi
doaj   +1 more source

Equilibrium Points in the Restricted Full Three Body Problem with Ellipsoidal Primaries

open access: yes, 2017
Equilibrium points in the Restricted Full Three Body Problem with ellipsoidal primaries are investigated. The approach adopted by some in the literature that approximates the primaries by means of the spherical harmonic potential is demonstrated to be ...
X. Xin, X. Hou
semanticscholar   +1 more source

Pharmacological inhibition of the PERK pathway modulates hepatocellular carcinoma growth and immune signaling

open access: yesFEBS Open Bio, EarlyView.
Pharmacological inhibition of PERK in a DEN‐induced mouse model of liver cancer does not reduce tumor burden but alters cellular stress signaling. Despite blocking PERK activity, downstream stress responses, including CHOP expression, remain active, suggesting compensatory mechanisms within the unfolded protein response that may influence tumor ...
Ada Lerma‐Clavero   +5 more
wiley   +1 more source

Mathematical modeling applied to understand the host-pathogen interaction of HIV infection in Bangladesh [PDF]

open access: yesSurveys in Mathematics and its Applications, 2017
The most urgent public health problem today is to devise effective strategies to minimize the destruction caused by the AIDS epidemic. The understanding of HIV infection through mathematical modeling have made a significant contribution.
S. K. Sahani   +2 more
doaj  

A yeast model of 5‐oxoproline accumulation reveals a general toleration to 5‐oxoproline

open access: yesFEBS Open Bio, EarlyView.
Using a yeast model, we show that even high accumulation of 5‐oxoproline causes only mild cellular stress and does not trigger oxidative stress. Instead, cells adapt by activating efflux pumps and diverse protective pathways, suggesting that previously proposed harmful effects of 5‐oxoproline may arise from indirect metabolic imbalances rather than the
Pratiksha Dubey   +4 more
wiley   +1 more source

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