Results 81 to 90 of about 271,459 (258)

Optimizing photoexcitation conditions for time‐resolved X‐ray solution scattering experiments

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering (TR‐XSS) is a powerful technique to visualize how proteins change their structure in real time after light activation. Selecting the right laser photoexcitation conditions—fluence, excitation geometry, and sample refresh rate—is critical to maximize the experimental signal while avoiding unwanted side effects ...
Matteo Levantino
wiley   +1 more source

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

open access: yesFEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli   +3 more
wiley   +1 more source

Dynamical environment in the vicinity of asteroids with an application to 41 Daphne

open access: yesResults in Physics, 2018
We studied the dynamical environment in the vicinity of the primary of the binary asteroid. The gravitational field of the primary is calculated by the polyhedron model with observational data of the irregular shape. The equilibrium points, zero-velocity
Yu Jiang
doaj   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

open access: yesFEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty   +13 more
wiley   +1 more source

Mathematical modeling applied to understand the host-pathogen interaction of HIV infection in Bangladesh [PDF]

open access: yesSurveys in Mathematics and its Applications, 2017
The most urgent public health problem today is to devise effective strategies to minimize the destruction caused by the AIDS epidemic. The understanding of HIV infection through mathematical modeling have made a significant contribution.
S. K. Sahani   +2 more
doaj  

Adenosine triphosphate as a modulator of protein interactions and stability

open access: yesFEBS Open Bio, EarlyView.
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

Equilibrium points in n -person games [PDF]

open access: yesProceedings of the National Academy of Sciences, 1950
Soit un jeu à de \(n\) personnes, où chaque joueur possède un systeme fini de stratégies pures. Chacun d'entre eux ayant choisi une tactique de jeu, l'ensemble de ces tactiques constitute un \(n\)-uple de stratégies pures. A tout \(n\)-uple correspond un système défini de paiement entre les partenaires. L'A. considère chacun de ces \(n\)-uples comme un
openaire   +3 more sources

Heterotropic regulation and negative homotropic cooperativity

open access: yesFEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea   +5 more
wiley   +1 more source

Home - About - Disclaimer - Privacy